风湿性心脏病患者外周血单个核细胞IL-22的表达及其意义  

Expression of IL-22 of the from Peripheral Blood Mononuclear Cells in Patients with Rheumatic Heart Disease

作　　者：段长恩 苏国宝[1] 周朝元[1] 韩培立[1] DUAN Chang-en;SU Guo-bao;ZHOU Chao-yuan(Department of Cardiovascular Surgery,the First Affiliated Hospital of Xinxiang Medical University & Henan Key Laboratory of Neurol Regeneration,Weihui 453100,China)

机构地区：[1]新乡医学院第一附属医院心血管外科/河南省神经修复重点实验室,河南卫辉453100

出　　处：《医学临床研究》2019年第8期1457-1459,共3页Journal of Clinical Research

基　　金：新乡医学院第一附属医院青年基金资助项目(QN-2017-B005).

摘　　要：【目的】探讨风湿性心脏病(RHD)患者外周血单个核细胞白细胞介素-22(IL-22)的表达及其意义。【方法】选取本院心血管外科2016年5月至2017年5月确诊的30例拟接受手术治疗的RHD患者为观察组,选择同期30例健康献血者为对照组。采用密度-梯度离心法获取两组受试者的外周血单个核细胞,并用ELISA法和qRT-PCR法分别检测两组外周血单个核细胞培养液上清中IL-22的含量及其对应细胞基因表达;扫描电镜观察与观察组外周血单个核细胞培养液上清共培养后的对照组外周血单个核细胞的形态变化。【结果】观察组外周血单个核细胞培养液上清中IL-22的含量及单个核细胞IL-22 mRNA相对表达量均明显高于对照组(P<0.05);随着与观察组外周血单个核细胞培养液上清液共培养时间的延长,对照组外周血单个核细胞的形态发生明显改变,甚至崩解破裂。【结论】RHD患者外周血单个核细胞过度表达IL-22,其在RHD的发生、发展中可能起着一定的促进作用。【Objective】To investigate the expression and significance of the interleukin-22 that secreted by the peripheral blood mononuclear cells from the patients with rheumatic heart disease (RHD) to provide theoretical basis for the clinical treatment.【Methods】A total of 30 patients with RHD who underwent surgery from May 2016 to May 2017 were selected as the observation group and 30 healthy blood donors were selected as the control group.Peripheral blood mononuclear cells from patients with rheumatic heart disease and healthy donors were isolated by density gradient centrifugation.The content and gene expression of IL-22 in the culture medium were detected using ELISA and qRT-PCR,respectively.At the same time,the cellular morphology of peripheral blood mononuclear were observed by scanning electron microscope after co-cultured with the culture medium from patients with rheumatic heart disease .【Results】The content and gene expression of IL-22 in the culture medium of patients with rheumatic heart disease were significantly higher than those in healthy donors.The differences were statistically significant(P<0.05).The morphology of peripheral blood mononuclear cells from healthy donors showed dramatically changed even collapsed and ruptured when they co-cultured with the secreted medium from RHD patients' mononuclear cells..【Conclusion】The over-expression of IL-22 of the peripheral blood mononuclear cells from patients with rheumatic heart disease plays a certain role in the occurrence and development of the disease RHD.

关 键 词：风湿性心脏病/病理生理学 细胞因子类 

分 类 号：R541.2[医药卫生—心血管疾病]