拟南芥膜定位蛋白At CP2调控抗冻性的功能分析  

Functional Analysis of Arabidopsis Membrane Locating Protein AtCP2 in Regulation of Cold Stress Resistance

作　　者：齐欣 丁庆倩 苏晨 徐伟亚 牟永莹 葛林豪 徐兆师[1] 陈隽[1] 潘映红[1] 马有志[1] 陈明[1] QI Xin;DING Qingqian;SU Chen;XU Weiya;MU Yongying;GE Linhao;XU Zhaoshi;CHEN Jun;PAN Yinghong;MA Youzhi;CHEN Ming(Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081;College of Agriculture, Northwest Agriculture and Forestry Science and Technology, Shaanxi Yangling 712100, China)

机构地区：[1]中国农业科学院作物科学研究所,农作物基因资源与基因改良国家重大科学工程,农业农村部麦类生物学与遗传育种重点实验室,北京100081 [2]西北农林科技大学农学院,陕西杨凌712100

出　　处：《中国农业科技导报》2019年第9期58-68,共11页Journal of Agricultural Science and Technology

基　　金：国家转基因生物新品种项目培育重大项目(2016ZX08002005);中国农业科学科技创自工程项目资助

摘　　要：COR家族成员在调控植物响应低温胁迫过程中发挥重要作用。从拟南芥中克隆一个新的低温胁迫响应基因At CP2,其属于COR基因家族中的COR413亚家族,编码区612 bp,编码203个氨基酸,分子量22.75k Da,等电点9.44。亚细胞定位和基因表达模式分析结果表明:At CP2定位在叶绿体膜上,对ABA、PEG、Na Cl和低温四种非生物胁迫均有响应。基因功能分析结果显示:功能缺失性突变体cp2冷处理后存活率明显高于野生型拟南芥(WT),相对电导率和MAD均低于WT,证明At CP2基因负向调控植物抗冻性。蛋白质组及qRT-PCR分析结果证明At CP2通过负向调控4个ABA及低温胁迫相关基因(包括ABA2、KIN1、COR47和COR15B)的表达影响植物抗冻性。总之,At CP2基因的功能分析结果对了解COR413基因家族的功能提供了参考。Members of COR family play important roles in regulating plant response to low temperature stress. In this study, a new cold stress response gene AtCP2 was cloned from Arabidopsis thaliana. AtCP2 belonged to COR413 subfamily of COR gene family. The coding region of AtCP2 gene was 612 bp and encoded 203 amino acids with a molecular weight of 22.75 kDa and an isoelectric point of 9.44. Subcellular localization and gene expression pattern analysis showed that AtCP2 was located on chloroplast membrane and respond to abiotic stresses such as ABA, PEG, NaCl and cold. The results of gene function analysis showed that the survival rate of cp2 mutant after cold treatment was significantly higher than that of wild-type (WT) Arabidopsis, and the relative conductivity and MAD were lower than that of WT, which indicated that AtCP2 gene negatively regulated plant cold stress resistance. The results of proteomic and qRT-PCR analysis showed that AtCP2 affected plant cold stress resistance by negatively regulating the expression of four ABA and cold stress-related genes (including ABA2, KIN1, COR47 and COR15B). In conclusion, the above analysis of AtCP2 gene provided basis for understanding the function of COR413 gene family.

关 键 词：拟南芥 At CP2基因 低温胁迫 蛋白质组 下游基因 

分 类 号：Q78[生物学—分子生物学]