_D-Proline capped gold nanoclusters for turn-on detection of serum Raltitrexed  

作　　者：Weifei Lan Qiqi Tan Juan Qiao Gangyi Shen Li Qi 

机构地区：[1]Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Bio-systems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China [2]School of Chemical Sciences,University of Chinese Academy of Sciences, Beijing 100049, China [3]Department of Chemical Engineering, Beijing Institute of Petro-chemical Technology, Beijing 102617, China [4]School of Pharmacy, Minzu University of China, Beijing 100081, China

出　　处：《Chinese Chemical Letters》2019年第9期1627-1630,共4页中国化学快报（英文版）

基　　金：the financial support from the National Natural Science Foundation of China (Nos. 21575144, 91732103, 21874138, 21635008, 21621062)

摘　　要：With _D-proline as the reducing and capping agent, fluorescent gold nanoclusters were rapidly prepared(_D-Pro@AuNCs) within 10 min at 100℃. In the present of gold nanoparticles, the fluorescence of _DPro@AuNCs was remarkably quenched. Interestingly, based on the electrostatic interaction between anticancer drug Raltitrexed and gold nanoparticles induced fluorescence "turn-on" principle, a high selective assay for detection of Raltitrexed was established with the probe associating the fluorescence emission at 435 nm. The fluorescence intensity of _D-Pro@AuNCs linearly correlated with the concentration of Raltitrexed in the range from 5.0 mmol/L to 40.0 mmol/L(R^2= 0.999) and the limit of detection was 1.9 mmol/L. Further, after Raltitrexed was abdominal injected in rats, a metabolic approach was constructed with the prepared fluorescent probe. It showed great potential of AuNCs-based sensing probes for application in analysis of serum anticancer drugs.With _D-proline as the reducing and capping agent, fluorescent gold nanoclusters were rapidly prepared(_D-Pro@AuNCs) within 10 min at 100℃. In the present of gold nanoparticles, the fluorescence of _DPro@AuNCs was remarkably quenched. Interestingly, based on the electrostatic interaction between anticancer drug Raltitrexed and gold nanoparticles induced fluorescence "turn-on" principle, a high selective assay for detection of Raltitrexed was established with the probe associating the fluorescence emission at 435 nm. The fluorescence intensity of _D-Pro@AuNCs linearly correlated with the concentration of Raltitrexed in the range from 5.0 mmol/L to 40.0 mmol/L(R^2= 0.999) and the limit of detection was 1.9 mmol/L. Further, after Raltitrexed was abdominal injected in rats, a metabolic approach was constructed with the prepared fluorescent probe. It showed great potential of AuNCs-based sensing probes for application in analysis of serum anticancer drugs.

关 键 词：D-Pro capped gold NANOCLUSTERS Fluorescent probe Turn on Sensing SERUM RTX 

分 类 号：O6[理学—化学]