中草药复方及其与抗生素联用对斜带石斑鱼河流弧菌病的治疗效果  

Effect of Chinese Medicines Formula and Antibiotics Preventing Vibrio fluvialis Diseases of Epinephelus coioides

作　　者：徐晓津 李秀华[4] 马一帆 李慧耀 祁欣 吕海龙 鄢庆枇 XU Xiao-jin;LI Xiu-hua;MA Yi-fang;LI Hui-yao;QI Xin;LYU Hai-long;YAN Qing-pi(State Key Laboratory of Large Yellow Croaker Breeding, Fujian Fuding Seagull Fishing Food Co.Ltd., Ningde, 352103, China;Fisheries College, Jimei University, Xiamen 361021, China;FujianTianma Science and Technology Group Co. Ltd., Fuzhou 350308, China;Department of TraditionalChinese Medicine, Second Affiliated Hospital of Xiamen Medical College, Xiamen 361021, China;College of Marine and Earth, Xiamen University, Xiamen 361005, China)

机构地区：[1]福建福鼎海鸥水产食品有限公司,大黄鱼育种国家重点实验室,福建宁德352103 [2]集美大学水产学院,福建厦门361021 [3]福建天马科技股份集团有限公司,福建福州350308 [4]厦门医学院附属第二医院中医科,福建厦门361021 [5]厦门大学海洋与地球学院,福建厦门3610051

出　　处：《广东海洋大学学报》2019年第5期38-43,共6页Journal of Guangdong Ocean University

基　　金：2018年开放课题基金项目(LYC2018RS04,闽海鸥[2018]31号);福建省自然基金项目(2018J01455、2016N5009);国家基金(是自然科学基金)(31702384);福建省科技重大专项(2016NZ0001-3);2017年集美大学研究生教育教学改革研究项目(集大研[2017]18号);教育部鳗鱼工程研究中心开放基金项目(RE201704)

摘　　要：【目的】通过药物体外抑菌试验及斜带石斑鱼抗病力研究,筛选有效治疗斜带石斑鱼(Epinephelus coioides)河流弧菌(Vibrio fluvialis)病复方中药与中西药联用配方。【方法】用黄连、乌梅、黄柏比例分别为0.9∶1.3∶0.9、1.2∶0.9∶1.0、1∶1∶1的复方中药对河流弧菌进行体外抑菌实验;将质量分数2.2%复方中草药与中西药联用复方分别添加到基础饲料中,投喂感染河流弧菌的斜带石斑鱼73 d,研究中西药对石斑鱼河流弧菌病治疗效果。其中,试验组Ⅰ、Ⅱ、Ⅲ的黄连、乌梅、黄柏比例分别为0.9∶1.3∶0.9、1.2∶0.9∶1.0、1∶1∶1,试验组Ⅳ、Ⅴ、Ⅵ的黄连、乌梅、黄柏、恩诺沙星配比分别为0.9∶1.3∶0.9∶1.3、1.2∶0.9∶1.0∶0.9、1∶1∶1∶1,另设空白对照组Ⅰ(健康鱼,投喂基础饲料)、Ⅱ(病鱼,投喂基础饲料)和阳性对照组Ⅲ(病鱼,投喂板黄散)、Ⅳ(病鱼,投喂大黄五倍子散)。【结果】黄连、乌梅、黄柏配比1∶1∶1、质量浓度200mg/mL的复方中药对河流弧菌的体外抑菌效果较佳,为最佳配方及用量。40 d病鱼死亡数,对照组Ⅱ>对照组Ⅳ>对照组Ⅲ>试验组Ⅰ>试验组Ⅲ>试验组Ⅱ>试验组Ⅴ>试验组Ⅵ>试验组Ⅳ>对照组Ⅰ,试验组Ⅱ的黄连、乌梅、黄柏比1.2∶0.9∶1.0为疗效最佳的中药复方,试验组Ⅳ的黄连、乌梅、黄柏、恩诺沙星比为0.9∶1.3∶0.9∶1.3为疗效最佳的中西药联用复方。【结论】中药复方及中西药联用复方均可较好治疗斜带石斑鱼河流弧菌病,后者药效优于前者。【Objective】 To study the effects of antibacterial effect of the drugs against Vibro fluvialis in vitro and the drugs on the resistance to diseases of Epinephelus coioides.To screen traditional Chinese medicines formula and Chinese and western medicines formula in preventing Ⅴ.fluvialis diseases of E.coioides.【Method】Bacteriostatic test on traditional Chinese medicines formula against Vibro fluvialis in vitro was used.The ratios of Huanglian, Prunus mume and Cortex Phellodendri of Chinese medicines formulas Ⅰ,Ⅱ,Ⅲ are 0.9∶1.3∶0.9, 1.2∶0.9∶1.0,1∶1∶1, respectively.To test the drugs in preventing the diseases, E.coioides was injected with either PBS as control or Ⅴ.fluvialis for treatment.The fishes were divided into 10 groups for 73 days: control groupⅠ(healthy fish), control groupⅡ(diseased fish), drug production in aquaculture control group Ⅲ(Banhuang San supplemental group, diseased fishes) and control group Ⅳ(Dahuang Wubeizi San supplemental group, diseased fishes).The ratios of Huanglian, Prunus mume and Cortex Phellodendri were different in Chinese medicines formulas trial groupⅠ,Ⅱ,Ⅲ.The ratio of Huanglian, Prunus mume, Cortex Phellodendri and enrofloxaci were different in Chinese and western medicines formulas trial groupⅣ,Ⅴ,Ⅵ.The groups with 3 replicates per treatment of feed containing the drugs feed additives at 2.2% respectively,were fed to Epinephelus coioides.【Result】The concentrations of Chinese medicines formulas Ⅲ(200 mg/ml) is the best formula.The mortality for 40 d in test groups were as follows: control groupⅡ> control group Ⅳ> control group Ⅲ> trial groupⅠ> trial group Ⅲ> trial groupⅡ> trial group Ⅴ> trial group Ⅵ> trial groupⅣ> control groupⅠ.The ratios of Huanglian, Prunus mume and Cortex Phellodendri of Chinese medicines formulaⅡis 1.2∶0.9∶1.0.The ratio of Huanglian, Prunus mume, Cortex Phellodendri and enrofloxaci in Chinese and western medicines formula Ⅳ are 0.9∶1.3∶0.9∶1.3.These are the best formulas.�

关 键 词：复方中药 中西药联用 恩诺沙星 斜带石斑鱼 河流弧菌 

分 类 号：S943.334.42[农业科学—水产养殖]