七氟醚预处理对局灶性脑缺血再灌注损伤大鼠海马血管紧张素Ⅱ-2受体及炎症的影响  

Effects of Sevoflurane Pretreatment on Angiotensin Ⅱ Receptor and Inflammation in Hippocampus of Rats with Focal Cerebral Ischemia-reperfusion Injury

作　　者：秦起 李芮 曾庆繁 QIN Qi;LI Rui;ZENG Qingfan(College of Anesthesiology, Guizhou Medical University, Guiyang 550004, Guizhou,China;Department ofAnesthesiology, Shiyan Taihe Hospital, Shiyan 442000, Hubei, China)

机构地区：[1]贵州医科大学麻醉学院,贵州贵阳550004 [2]十堰市太和医院麻醉科,湖北十堰442000

出　　处：《贵州医科大学学报》2019年第9期1024-1028,共5页Journal of Guizhou Medical University

基　　金：贵州省科技厅基金项目(TN2014-75)

摘　　要：目的:观察七氟醚预处理对局灶性脑缺血再灌注损伤大鼠海马血管紧张素Ⅱ-2受体(AT 2R)及炎症的影响。方法:健康雄性SD大鼠80只,随机均分成对照组(Sham组)、脑缺血再灌注组(IR组)、七氟醚+脑缺血再灌注组(SP组)、AT 2R拮抗剂+七氟醚+脑缺血再灌注组(SPD组)及AT 2R拮抗剂+脑缺血再灌注组(PD组),再灌注24 h后行神经功能缺陷评分,TTC染色并检测海马组织AT 2R、TNF-a及IL-1β含量。结果:与Sham组比较,IR组、SP组、SPD组及PD组大鼠神经功能缺陷评分及脑梗死体积升高,AT 2R、TNF-a及IL-1β含量升高,差异有统计学意义( P <0.05);与IR组比较,SP组大鼠神经功能缺陷评分及脑梗死体积降低,AT 2R明显上调,TNF-a、IL-1β含量降低,差异有统计学意义( P <0.05);与SP组比较,SPD组大鼠神经功能缺陷评分、脑梗死体积和TNF-a、IL-1β含量升高,AT 2R表达降低,差异有统计学意义( P <0.05)。结论:七氟醚预处理对局灶性脑缺血再灌注损伤的保护作用可能与激活海马AT 2R、减轻海马组织炎症有关。Objective: To investigate the effect of sevoflurane pretreatment on angiotensin II receptor and inflammation in hippocampus of rats with focal cerebral ischemia-reperfusion injury. Methods: Eighty healthy male SD rats were randomly divided into control group (Sham group), cerebral ischemia-reperfusion group (IR group), sevoflurane cerebral ischemia-reperfusion group (SP group), AT2R antagonist sevofluranecerebral ischemia-reperfusion group (SPD group) and AT2R antagonist cerebral ischemia-reperfusion group (PD group). Neurological deficit score, TTC staining and IL-1 β content in hippocampal tissue were measured 24 hours after reperfusion. Results: In comparison with that sham group, the neurological deficit score and the volume of cerebral infarction in the IR, the SP, the SPD and the PD group were increased. The contents of AT2R,TNF-a and IL-1 β increased, the difference was statistically significant ( P< 0.05). Compared with IR group, the score of neurological deficit and the volume of cerebral infarction in SP group were decreased, and AT2R was significantly up-regulated. The content of TNF-a, IL-1 β was decreased, and the difference was statistically significant (P <0.05). Compared with SP group, the neurological deficit score, cerebral infarction volume and the content of TNF-a and IL-1 β in SPD group were increased. The expression of AT2R decreased , and the difference was statistically significant ( P <0.05). Conclusion: The protective effect of sevoflurane pretreatment on focal cerebral ischemia-reperfusion injury is related to the activation of hippocampal AT2R, to reduce inflammation in hippocampal tissue.

关 键 词：七氟醚 脑缺血预处理 再灌注损伤 血管紧张素Ⅱ-2受体 

分 类 号：R614.2[医药卫生—麻醉学]