RGD靶向超声造影剂对大鼠2期压力性损伤创面的靶向性研究  

Targeting effect of RGD-targeted ultrasound contrast agent in rats with stage 2 pressure injury wounds

作　　者：闫常帅 陈江琼 王笑一 胡芳[1] 张杰[1] YAN Chang-shuai;CHEN Jiang-qiong;WANG Xiao-yi;HU Fang;ZHANG Jie(Department of General Surgery, Tianjin Medical University General Hospital, Tianjin 300052, China;the First Affiliated Hospital of Chongqing Medical University;the Second Hospital of Tianjin Medical University)

机构地区：[1]天津医科大学总医院普外科,300052 [2]重庆医科大学第一附属医院 [3]天津医科大学第二医院

出　　处：《天津医药》2019年第9期932-936,共5页Tianjin Medical Journal

基　　金：天津医科大学天津市高等学校科研业务费资助项目（2017KJ196）

摘　　要：目的检测大鼠2期压力性损伤模型中整合素αvβ3的表达情况,制备精氨酸-甘氨酸-天冬氨酸(RGD)靶向超声造影剂,观察其对大鼠2期压力性损伤模型的靶向性。方法将36只大鼠随机分为实验组、对照组,每组18只。实验组采用皮肤桥法建立2期压力性损伤模型,对照组不做处理。成模后HE染色观察2组大鼠皮肤组织病理变化,免疫组化染色检测整合素αvβ3的表达情况。然后实验组大鼠分别使用自制的RGD靶向超声造影剂和空白造影剂评估对创面的靶向效果。结果实验组皮肤HE染色显示损伤达真皮层,对照组未见损伤。实验组新生的毛细血管内皮细胞和表皮细胞内整合素αvβ3表达呈阳性,对照组呈阴性。实验组大鼠注射RGD靶向超声造影剂后与空白造影剂相比靶向黏附值明显增高(P<0.01)。结论整合素αvβ3在大鼠2期压力性损伤中呈阳性表达,将其作为靶向位点制备RGD靶向超声造影剂对2期压力性损伤具有良好的靶向性。Objective To detect integrin αVβ3 expression in rat model of stage 2 pressure injury, and prepare arginineglycine- aspartic acid (RGD)-targeted ultrasound contrast agent (CA), thus exploring its targeting effect for skin lesions. Methods Thirty-six rats were randomly divided into experimental group and control group, with 18 rats in each group. Rats were established stage 2 pressure injury models by skin bridge method for experimental group, while rats of the control group did not do any treatment. After the model was formed, the pathological changes of skin tissue were observed by HE staining. The integrin αvβ3 was detected by immunohistochemistry. Then, the self-made RGD-targeted ultrasound CA and the blank counterpart were used to evaluate the wound in experimental group. Results HE staining showed that the damage reached the dermis layer in experimental group, and no damage was found in the control group. The expressions of integrin αVβ3 were positive in the capillary endothelial cells and epidermal cells of the experimental group, and which were negative in the control group. The targeted adhesion value was significantly higher after injecting RGD-targeted ultrasound contrast agent in the experimental group than that of blank contrast agent group (P<0.01). Conclusion The expression of integrin αVβ3 is positive in the rat model of stage 2 pressure injury, suggesting that integrin αvβ3 can be used as a target site to prepare RGD-targeted ultrasound contrast agent. Integrin αVβ3 shows a good targeting effect and can be used as a new imaging method for diagnosing and treating pressure injury.

关 键 词：整合素ΑVΒ3 造影剂 超声造影 压力性损伤 RGD肽 

分 类 号：R47[医药卫生—护理学]