白细胞介素-35基因多态性与冠心病患者认知功能障碍的相关性研究  

Association between interleukin-35 polymorphisms and the risk of cognitive impairment in patients with coronary heart disease

作　　者：施莹[1] 杨子聪[1] 刘海润[2] 薛焱[1] 张舒 黄信顺 卢建勇 刘伶[1] SHI Ying;YANG Zi-cong;LIU Hai-run;XUE Yan;ZHANG Shu;HUANG Xin-shun;LU Jian-yong;LIU Ling(Department of Cardiology,the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China;the Center of Cognitive and Sleeping, the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China)

机构地区：[1]广西壮族自治区人民医院心血管内科,530021 [2]广西壮族自治区人民医院认知睡眠中心,530021

出　　处：《天津医药》2019年第9期947-952,共6页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目（81460061）;广西医疗卫生适宜技术研究与开发项目（S201546）

摘　　要：目的探讨白细胞介素(IL)-35基因多态性与冠心病患者认知功能障碍发生风险的相关性。方法选择2015年2月-2016年10月在广西壮族自治区人民医院心血管内科首次诊断为冠心病的患者349例,均使用简易精神状态检查量表(MMSE)进行认知功能评估,按评估结果分为认知功能正常组(对照组)205例和认知功能损伤组(病例组)144例。使用Sequenom Mass Array系统对IL-35单核苷酸多态性(SNPs)位点(rs582054、rs2243115、rs428253、rs583911、rs568408和rs4740)进行基因型分析。使用酶联免疫吸附实验(ELISA)检测血浆IL-35水平。其他相关指标均由广西壮族自治区人民医院检验科按标准流程进行检测。采用Logistic回归模型探讨IL-35 SNPs与冠心病患者认知功能障碍发生风险的相关性。结果 2组间血浆IL-35水平差异无统计学意义[42.26(17.19,203.06)ng/L vs.40.60(22.69,105.65)ng/L,Z=0.384,P>0.05]。Logistic回归分析结果显示,校正吸烟史、饮酒史、高血压、糖尿病病史、认知功能障碍家族史、年龄、丙氨酸转氨酶(ALT)及空腹血糖水平等因素后,并未发现IL-35多态性位点(rs582054、rs2243115、rs428253、rs583911、rs568408和rs4740)与冠心病患者认知功能障碍发生风险存在相关性。结论IL-35基因SNPs与冠心病患者认知功能障碍发生风险之间不存在相关性,将来仍需进一步扩大样本量并对其可能的机制进行探讨。Objective To explore the association between interleukin-35(IL-35) polymorphisms and the risk of cognitive impairment in patients with coronary heart disease (CHD). Methods A total of 349 patients diagnosed as CHD in our hospital from February 2015 to October 2016 were enrolled in our case-control study. The cognitive function was evaluated by mini-mental state examination (MMSE). Patients were divided into control group (n=205) and cognitive impairment group (n=144) according to the MMSE results. Sequenom Mass array was used to analyze the genotypes of IL-35 SNPs (rs582054, rs2243115, rs428253, rs583911, rs568408 and rs4740), and ELISA was used to detect the plasma level of IL-35, while other parameters were examined by standard protocol in the laboratory of the People’s Hospital of Guangxi Zhuang autonomous region. The binary Logistic regression model was used to reveal the associations between IL-35 polymorphisms and the risk of cognitive impairment in CHD. Results There was no significant difference in plasma level of IL-35 between two groups [42.26 (17.19, 203.06) ng/L vs. 40.60 (22.69, 105.65) ng/L, Z=0.384, P>0.05]. No significant association was found between IL-35 polymorphisms and the risk of cognitive impairment in CHD patients. After the adjustment of risk factors, no significant association was found between IL-35 polymorphisms and the risk of cognitive impairment in CHD patients. Conclusion There is no relationship between IL-35 polymorphisms and the risk of cognitive impairment in CHD patients. It is still necessary to expand the sample size and explore its mechanism in the future.

关 键 词：白细胞介素-35 多态性 单核苷酸 冠心病 认知功能障碍 

分 类 号：R541.4[医药卫生—心血管疾病]