ICU多重耐药铜绿假单胞菌产氨基糖苷类修饰酶基因的研究  

Study on the production of aminoglycoside-modifying enzyme gene from ICU multidrug-resistant Pseudomonas aeruginosa

作　　者：彭咏麟 刘立君 PENG Yonglin;LIU Lijun(Department of Clinical Laboratory,Hengyang Central Hospital,Hengyang,Hunan 421001,China)

机构地区：[1]湖南省衡阳市中心医院检验科

出　　处：《检验医学与临床》2019年第18期2607-2609,共3页Laboratory Medicine and Clinic

基　　金：湖南省卫生和计划生育委员会基金项目(湘卫计委B2015-158)

摘　　要：目的 探讨重症监护室多重耐药铜绿假单胞菌产氨基糖苷类修饰酶基因的表达.方法 采用PCR法检测60株多重耐药铜绿假单胞菌的4种氨基糖苷类修饰酶基因ant(2″)-Ⅰ 、ant(3″)-Ⅰ 、anc(6')-Ⅰ和anc(6')-Ⅱ.结果 60株多重耐药铜绿假单胞菌中检测到含有氨基糖苷类修饰酶基因的菌株共16株,检出率为26.67%(16/60);8株含ant(2″)-Ⅰ基因,检出率为13.33%(8/60);10株含有anc(6')-Ⅱ基因,检出率为16.67%(10/60);其中两株同时含ant(2″)-Ⅰ基因和anc(6')-Ⅱ基因,未检测出ant(3″)-Ⅰ和anc(6')-Ⅰ基因.结论 重症监护室多重耐药铜绿假单胞菌中氨基糖苷类修饰酶基因的表达率较高,氨基糖苷类修饰酶介导的耐药在多重耐药铜绿假单胞菌的耐药机制中占有重要的地位.Objective To investigate the expression of aminoglycoside-modifying enzyme gene produced by multidrug-resistant Pseudomonas aeruginosa (P.aeruginosa) in ICU. Methods Four aminoglycoside modifying enzyme genes [ant(2″)-Ⅰ,ant(3″)-Ⅰ,anc(6′)-Ⅰand anc(6′)-Ⅱ] of 60 multidrug-resistant P.aeruginosa were detected by PCR. Results A total of16 strains containing aminoglycoside modifying enzyme gene were detected in 60 multidrug-resistant P.aeruginosa strains,and the rate was26.67%(16/60).And 8 strains (13.33 %) contained ant(2″)-Ⅰ gene,10 strains (16.67%) contained the anc(6′)-Ⅱ gene,2 of which contained both the ant(2″)-Ⅰ gene and the anc(6′)-Ⅱ gene,and ant(3″)-Ⅰ and anc(6′)-Ⅰ gene were not detected. Conclusion The multidrug-resistant P.aeruginosa in the ICU of this region has a high expression rate of aminoglycoside-modifying enzyme gene,and drug resistance resulted by aminoglycoside-modifying enzyme plays an important role in the resistance mechanism of multidrug-resistant P.aeruginosa.

关 键 词：铜绿假单胞菌 PCR 氨基糖苷类修饰酶 重症监护室 

分 类 号：R446.5[医药卫生—诊断学]