微氧条件下自养-异养联合反硝化工艺的电子平衡分析  

Electron Equilibrium Analysis of Integrated Autotrophic and Heterotrophic Denitrification Process Under Micro-aerobic Conditions

作　　者：张若晨 陈川 邵博 王威 徐熙俊 任南琪 ZHANG Ruo-chen;CHEN Chuan;SHAO Bo;WANG Wei;XU Xi-jun;REN Nan-qi(State Key Laboratory of Urban Water Resource and Environment,School of Environment,Harbin Institute of Technology,Harbin 150090,China)

机构地区：[1]哈尔滨工业大学环境学院城市水资源与水环境国家重点实验室

出　　处：《环境科学》2019年第9期4128-4135,共8页Environmental Science

基　　金：国家重点研发计划项目(2016YFC0401102-2);国家自然科学基金项目(51576057,51676057);中国博士后科学基金项目(AUGA 4130903217);黑龙江省博士后科学基金项目(AUGA 4110002617);城市水资源与环境国家重点实验室(哈尔滨工业大学)项目(2015DX04)

摘　　要：自养-异养联合反硝化(integrated autotrophic and heterotrophic denitrification,IAHD)工艺可以同时进行硫化物,硝酸盐和有机物的降解,作为工业废水处理的关键单元近年来受到广泛关注.引入微量氧气作为电子受体的微氧技术已被证明是强化IAHD运行效能的有效策略.本研究关注于IAHD工艺的电子平衡计算并发现了IAHD生物反应器在微氧条件下运行时利用有限的硝酸盐可将硫化物和乙酸盐完全转化去除.在IAHD序批实验中,当电子缺失率达到峰值55.1%时,硫化物、硝酸盐和乙酸盐去除效率和去除速率均最高.进一步的硫化物氧化间歇实验表明,电子得失不平衡现象发生在生物硫化物氧化过程中,当氧气含量为5mL和10mL时,电子缺失率分别为18.7%和38.2%.Illumina微生物群落测序结果表明,Thiobacillus、Thauera、Mangroviflexus和Erysipelothrix为硫氧化过程的主要占优属,其中Thiobacillus的相对丰度随着电子缺失率的增加而增加.本研究揭示了微氧条件下电子受体缺失现象与强化的IAHD运行效能之间的潜在联系,并为深入探讨硫、氮和有机碳的代谢机制提供了新的研究视角.The integrated autotrophic and heterotrophic denitrification (IAHD) process,which can simultaneously degrade sulfide, nitrate,and organic carbon with nitrate as a solo electron acceptor,has gained increasing attention as a key unit in industrial wastewater treatment.Micro-aerobic technology,which introduces trace oxygen as an additional electron acceptor,has been demonstrated as an effective strategy for enhancing the IAHD performance.This study focus on the electronic balance calculation of the IAHD process and reveals for the first time that the IAHD process can efficient proceed with an insufficient supply of electron acceptors (nitrate) under micro-aerobic conditions.In the IAHD batch tests,the highest sulfide,nitrate,and acetate removal efficiencies and rates were obtained with an electronic deletion rate peak at 55.1%.Further sulfide oxidizing batch tests demonstrated that the electronic deletion rates were 18.7% and 38.2% under oxygen contents of 5 mL and 10 mL,respectively,in the biological sulfide oxidizing process. Illumina sequencing was used to analyze the microbial community structure in the sulfide oxidation process and indicated Thiobacillus, Thauera,Mangroviflexus,and Erysipelothrix dominated in all community compositions,in which the relative abundance of Thiobacillus increased with an increase in the electronic deletion rate.This study reveals a potential linkage between the electronic gap and the enhanced IAHD performance,which proves new insights into the simultaneous sulfur,nitrogen,and organic carbon removal process.

关 键 词：自养-异养联合反硝化(IAHD) 微氧 硫氧化 电子受体 微生物群落结构 

分 类 号：X703[环境科学与工程—环境工程]