氧化苦参碱通过PTEN/PI3K/Akt/mTOR通路对人膀胱癌T24细胞增殖、凋亡的影响研究  

A Study on Effect of Oxymatrine on Proliferation and Apoptosis of T24 Human Bladder Cancer Cells Through PTEN/PI3K/Akt/mTOR Pathways

作　　者：孙超群[1] 杨树立[1] 陈桂平[1] 郝阳 莫承强[2] SUN Chaoqun;YANG Shuli;CHEN Guiping;HAO Yang;MO Chengqiang

机构地区：[1]平顶山市第一人民医院泌尿外科,河南平顶山467000 [2]中山大学附属第一医院泌尿外科,广东广州510080

出　　处：《新中医》2019年第9期9-12,共4页New Chinese Medicine

基　　金：2018年河南省医学科技攻关计划（2018020938）

摘　　要：目的:探讨氧化苦参碱能否通过第10号染色体缺失的磷酸酶及张力蛋白同源的基因/3-磷酸肌醇激酶/蛋白激酶B/哺乳动物雷帕霉素靶向基因(Phosphatase and tension homolog deleted on chromosome ten/phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin,PTEN/PI3K/Akt/mTOR)通路对人膀胱癌T24细胞的增殖、凋亡产生影响。方法:T24细胞复苏传代后与不同剂量氧化苦参碱共培养,MTT法检测不同剂量氧化苦参碱对T24细胞增殖的影响,流式细胞仪检测氧化苦参碱对T24细胞凋亡的影响,Hoechst光镜观察氧化苦参碱对T24细胞核形态的影响,Western blot检测氧化苦参碱对T24细胞PI3K、Akt、mTOR、PTEN蛋白表达的影响。结果:与0μmol/L组比较,氧化苦参碱各剂量组T24细胞的增殖率下降,凋亡率升高,PI3K、Akt、mTOR蛋白表达下降,PTEN蛋白表达上升,且在一定范围内存在剂量依赖性(P<0.05)。结论:氧化苦参碱可能通过PTEN/PI3K/Akt/mTOR通路抑制T24细胞的增殖,促进其凋亡。Objective:To discuss whether oxymatrine can exert effect on proliferation and apoptosis of T24 human bladder cancer cells through phosphatase and tension homolog deleted on chromosome ten/phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin(PTEN/PI3 K/Akt/mTOR) pathways. Methods:T24 cells were co-cultured with oxymatrine of different doses after resuscitation and passage. The effect of oxymatrine of different doses on proliferation of T24 cells was detected by MTT assay;the effect of oxymatrine on apoptosis of T24 cells was detected by flow cytometry;the effect of oxymatrine on nuclear morphometry of T24 cells was given a light microscopic observation by Hoechst;the effect of oxymatrine on protein expressions of PI3 K,Akt,m TOR and PTEN of T24 cells was detected by Western blot. Results:Compared with those in the group of 0 μmol/L,the proliferation rates of T24 cells and the protein expressions of PI3 K, Akt and m TOR in the oxymatrine groups of each dose were decreased, while the apoptosis rates and protein expressions of PTEN were increased, and in a dose-dependent manner in a certain range(P < 0.05).Conclusion:Oxymatrine may inhibit the proliferation and promote the apoptosis of T24 cells through PTEN/PI3 K/Akt/mTOR pathways.

关 键 词：氧化苦参碱 人膀胱癌T24细胞 PTEN/PI3K/Akt/mTOR通路 细胞实验 

分 类 号：R285.5[医药卫生—中药学]