金边杂种胡颓子叶色突变体叶色变化机理的初步研究  

Preliminary study on leaf color change of a new leaf color mutant of Elaeagnus×ebbingei ‘Gilt Edge’

作　　者：陈红[1] 吴昌琦 高若诚 刘志强 李云龙[1] 王传永[1] 李丹丹[1] 陆小清[1] Chen Hong;Wu Changqi;Gao Ruocheng;Liu Zhiqiang;Li Yunlong;Wang Chuanyong;Li Dandan;Lu Xiaoqing(Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China;College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]江苏省中国科学院植物研究所(南京中山植物园),江苏南京210014 [2]南京农业大学园艺学院,江苏南京210095

出　　处：《江苏林业科技》2019年第4期49-53,共5页Journal of Jiangsu Forestry Science & Technology

基　　金：江苏省林业科技创新与推广项目“金边胡颓子、蓝莓的离体快繁技术研究及示范推广”(LYKJ[2017]39);南京市产学研合作后补助项目“金边胡颓子的彩色机理研究及推广利用”(201722087)

摘　　要：以野生型杂种胡颓子(WT)和突变型金边杂种胡颓子(MT)的3年生扦插苗为供试材料,研究突变型金边杂种胡颓子叶绿素含量与光合特性等生理指标,分析叶色变化的原因。结果显示:(1)叶绿素总含量:杂种胡颓子(WT1.530mg/g)>金边杂种胡颓子叶片绿色部位(MT-绿色1.078mg/g)>金边杂种胡颓子叶片黄色部位(MT-黄化0.031mg/g);类胡萝卜素含量:WT(0.505mg/g)>MT-绿色(0.318mg/g)>MT-黄化(0.050mg/g);(2)金边杂种胡颓子叶片绿色部位的Chla/b比值较WT叶片绿色部位,没有显著性的差异,但其叶片黄化部位的Chla/b比值显著低于野生型杂种胡颓子;(3)金边杂种胡颓子较野生型胡颓子光合速率及相关参数都有显著的改变。可见,金边杂种胡颓子的叶色变化是由叶片色素含量变化导致,导致植株的光合参数与叶绿素荧光参数等指标发生改变。The three-year-old cuttings of Elaeagnus×ebbingei‘Gilt Edge’wild type(WT)and mutant(MT)were used as materials to study the physiological parameters such as chlorophyll content and photosynthetic characteristics of mutant and to analyze leaf color change reason.The results showed that:(1)As for the total chlorophyll content,E.×ebbingei‘Gilt Edge’(WT 1.530 mg/g)>green part of mutant(MT-green 1.078 mg/g)>yellow part of mutant(MT-yellow 0.031 mg/g);as for carotenoid content,WT(0.505 mg/g)>MT-green(0.318 mg/g)>MT-yellow(0.050 mg/g);(2)There was no significant difference in the Chl a/b ratio between the green parts of MT leaves and the leaves of WT,but the Chl a/b ratio of the yellow parts of MT was significantly lower than that of WT hybrids;(3)The photosynthetic rate and related parameters of MT had significantly changed compared with those of WT.In conclusion,the leaf color change of MT was caused by change in the pigment content of leaves,which led to change in the photosynthetic parameters and chlorophyll fluorescence parameters of the plants.

关 键 词：胡颓子 叶绿素 叶色 光合速率 叶绿素荧光参数 

分 类 号：Q945.11[生物学—植物学] Q949.761.2