花生VAMP基因家族全基因组鉴定及表达分析  

Genome-Wide Identification and Expression Analysis of Peanut VAMP Gene Family

作　　者：陆晓东 张浩[2] 苑翠玲 孙全喜[2] 闫彩霞[2] 赵小波[2] 王娟[2] 李春娟[2] 郑奕雄 单世华[2] Lu Xiaodong;Zhang Hao;Yuan Cuiling;Sun Quanxi;Yan Caixia;Zhao Xiaobo;Wang Juan;Li Chunjuan;Zheng Yixiong;Shan Shihua(College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Shandong Peanut Research Institute,Qingdao 266100,China)

机构地区：[1]仲恺农业工程学院农业与生物学院,广东广州510225 [2]山东省花生研究所,山东青岛266100

出　　处：《山东农业科学》2019年第9期42-49,共8页Shandong Agricultural Sciences

基　　金：泰山学者特聘专家(ts201712080);山东省农业良种工程项目(2017LZN033,2017LZGC003);山东省现代农业产业技术体系(SDAIT-04-02);国家自然科学基金青年科学基金项目(31601336);广东省农业农村厅科技项目(2019KJ136);山东省重点研发计划项目(2016GGH4518);青岛市科技惠民计划项目(17-3-3-49-nsh)

摘　　要：植物囊泡结合膜蛋白(VAMP)是定位在囊泡上的运输蛋白,在植物发育以及响应生物和非生物胁迫中发挥重要作用。本研究对花生VAMP基因进行了全基因组鉴定与分析,并对它们在22个组织中的表达模式进行了分析。结果表明,二倍体野生种花生Arachis duranensis基因组有18个VAMP基因,Arachisi paensis基因组有21个,栽培种有41个,剔除假基因后花生VAMP基因家族有62个成员;表达模式分析表明AdVAMP17与AiVAMP1在大部分组织中均有表达,AdVAMP18与AiVAMP21在雌蕊中表达量最高,可能有特异性表达。本研究为进一步分析VAMP家族成员,并深入探讨其在花生中的生物学功能和进化模式奠定了基础。Vesicle associated membrane protein(VAMP) is a transport protein located on vesicles of plants, which plays an important role in plant development and response to biotic and abiotic stresses. In this study, the genome-wide of peanut VAMP gene was identified and analyzed, and the expression patterns in 22 tissues were analyzed. The results showed that there were 62 members in peanut VAMP gene family, 18 of which were the Arachis duranensis genomes, 21 of which were the A. ipaensis genomes and 41 of which were the cultivars. Expression pattern analysis showed that AdVAMP17 and AiVAMP1 were expressed in most tissues, and AdVAMP18 and AiVAMP21 had the highest expression level in pistils, which might have specific expression. These results laid a foundation for further analysis of the VAMP family members and discussing their biological functions and evolutionary patterns in peanut.

关 键 词：花生 囊泡结合膜蛋白(VAMP)基因家族 囊泡运输 表达模式 

分 类 号：S565.2[农业科学—作物学] Q781[生物学—分子生物学]