多指标综合评定肉豆蔻挥发油缓解慢性炎性疼痛的谱效关系  

Multi-Index Integrated Evaluation on the Spectrum Effect Relationship of Volatile Oil from Nutmeg on Relieving Chronic Inflammatory Pain

作　　者：肖俊勇[1] 李力 从仁怀[1] 刘宏 XIAO Junyong;LI Li;CONG Renhuai;LIU Hong(Joint Laboratory for the Research of Modern Preparation Technology,Huazhong University of Science and Technology and Infinitus,Wuhan 430075,China;WuXi Apptec (Wuhan)Co.,Ltd.,Wuhan 430075,China;National Engineering Research Center for Nanomedcine,Wuhan 430075,China)

机构地区：[1]华中科技大学无限极现代制剂技术联合实验室,武汉430075 [2]武汉药明康德新药开发有限公司,武汉430075 [3]国家纳米药物工程技术研究中心,武汉430075

出　　处：《医药导报》2019年第10期1264-1271,共8页Herald of Medicine

摘　　要：目的研究肉豆蔻挥发油缓解慢性炎性疼痛作用的谱效关系。方法采用气相色谱-质谱(GC-MS)联用技术建立19批肉豆蔻挥发油指纹图谱;取132只Wistar大鼠建立完全弗氏佐剂型慢性炎性疼痛模型,随机分为空白对照组、模型对照组、双氯芬酸钠(阳性对照)组和肉豆蔻挥发油组(19组分别为S1~S19,30 mg·kg^-1)。空白对照组及模型对照组每天灌胃给予等体积0.9%氯化钠溶液,双氯芬酸钠组每天灌胃给予相应剂量的双氯芬酸钠,肉豆蔻挥发油组每天灌胃给予相应剂量的肉豆蔻挥发油,分别检测足趾肿胀程度、辐射热痛觉阈值、第五腰椎背根神经节中NK-1R、环氧化酶(COX)-2的表达及血液中P物质含量;采用灰色关联度分析法对肉豆蔻挥发油GC-MS指纹图谱与各药效评价指标和总药效进行综合关联分析。结果肉豆蔻挥发油具有缓解慢性炎性疼痛的作用。26个特征共有峰的关联度均大于0.6,其中关联度最高的前五位峰为6号峰>8号峰>9号峰>5号峰>12号峰。结论肉豆蔻挥发油缓解慢性炎性疼痛作用是其有效物质成分群--26个特征共有峰代表的化学成分共同作用的结果,为以有效成分为基础的中药材质量控制标准的建立提供参考依据。Objective To investigate the spectrum effect relationship of nutmeg volatile oil on relieving chronic inflammatory pain. Methods The fingerprints of 19 species of volatile oil obtained from nutmeg were established by GC-MS;Complete Freund’s Adjuvant was used to establish chronic inflammatory pain model of Wistar rats.132 rats were randomly divided into blank control group, model control group, diclofenac sodium(positive control) group and nutmeg volatile oil group(19 groups:S1-S19,30 mg·kg^-1).The blank control group and model control group were given equal volume of 0.9% sodium chloride solution, diclofenac sodium group was given corresponding dose of diclofenac sodium, and nutmeg volatile oil group was given corresponding dose of nutmeg volatile oil.Joint swelling degree, thermal pain threshold, expression of NK-1 R and COX-2 in the fifth lumbar dorsal root ganglion and substance P in blood were detected to study the pharmacodynamics of nutmeg volatile oil on relieving chronic inflammatory pain.GC-MS fingerprints of nutmeg volatile oil with the efficacy evaluation indicators and total drug efficacy were analyzed by grey relational analysis. Results The nutmeg volatile oil had the effect of relieving chronic inflammatory pain.All of the correlations of 26 common peaks were greater than 0.6.Among them, the top five peaks with the highest correlation degree were No.6 > No.8 > No.9 > No.5 > No.12. Conclusion The effect of nutmeg volatile oil on relieving chronic inflammatory pain is the result of the joint action of its active ingredient group--the chemical components represented by the common peaks of 26 characteristics, which provides a reference basis for the establishment of quality control standards of Chinese medicinal materials based on active ingredients.

关 键 词：肉豆蔻挥发油 炎性疼痛 慢性 谱效关系 灰色关联度分析 

分 类 号：R932[医药卫生—生药学] R965[医药卫生—药学]