绞股蓝总苷分散片中绞股蓝皂苷A含量测定  

Determination of Gypenoside A in Gypenoside Dispersible Tablets

作　　者：徐文峰[1] 金鹏飞[1] 徐硕[1] 邝咏梅[1] 何笑荣[1] XU Wenfeng;JIN Pengfei;XU Shuo;KUANG Yongmei;HE Xiaorong(Departmentof Pharmacy,Beijing Hospital,National Center of Gerontology,Assessment of Clinical Drugs Risk and Individual Application Key Laboratory,Bejing 100730,China)

机构地区：[1]北京医院药学部国家老年医学中心药物临床风险与个体化应用评价北京市重点实验室

出　　处：《医药导报》2019年第10期1314-1316,共3页Herald of Medicine

基　　金：北京医院科技新星项目(BJ-2016-039)

摘　　要：目的建立高效液相色谱法测定绞股蓝总苷分散片中绞股蓝皂苷A的含量。方法采用Alltima C18色谱柱(150 mm×4.6 mm,5μm),流动相:乙腈-水(31:69),流速:1.0 mL·min^-1,检测波长:210 nm,进样体积20μL。结果制剂辅料不干扰绞股蓝皂苷A的测定,绞股蓝皂苷A在32.69~163.46μg·mL^-1的范围内线性良好,相关系数为1.000 0,精密度和稳定性良好,RSD均小于1.0%,绞股蓝皂苷A的平均加样回收率为97.2%(n=6);运用该方法测得3批绞股蓝总苷分散片中绞股蓝皂苷A的含量分别为4.05,3.88和3.21 mg·g^-1。结论该方法准确,简便,专属性强,可用于绞股蓝总苷分散片中绞股蓝皂苷A的含量测定。Objective To establish a high performance liquid chromatography(HPLC) method for content determination of gypenoside A in Gypenoside dispersible tablets. Methods An Alltima C18 column(150 mm× 4.6 mm,5 μm) was used for the separation with mobile phase of acetonitrile-purified water(31:69) at a flow rate of 1.0 mL·min-1.The detection wavelength was 210 nm and the injection volume was 20 μL. Results The excipients did not interfere with the determination of gypenoside A.The linear range of gypenoside A was 32.69-163.46 μg·mL^-1 with 1.000 0 as the correlation coefficient.The precision and stability were satisfactory with the relative standard deviations(RSDs) below 1.0%.The average spiked recovery of gypenoside A was 97.2%(n = 6).The determination results of three batches of gypenoside dispersible tablets were 4.05, 3.88 and 3.21 mg·g^-1. Conclusion The established method is accurate and simple with high specificity, and suitable for determination of gypenoside A in gypenoside dispersible tablets.

关 键 词：绞股蓝皂苷A 绞股蓝总苷分散片 含量测定 色谱法 高效液相 

分 类 号：R286[医药卫生—中药学] R927.2[医药卫生—中医学]