肢体缺血预处理对大鼠肝脏缺血再灌注损伤的影响  

Effects of limb ischemic preconditioning on hepatic ischemia reperfusion injury in rats

作　　者：余伟 黄长山 王谦 黄涛 丁月超 马超 Yu Wei;Huang Changshan;Wang Qian;Huang Tao;Ding Yuechao;Ma Chao(Department of Hepatobiliary & Pancreatic Surgery,Affiliated Tumor Hospital of zhengzhou university,Zhengzhou,453003,China)

机构地区：[1]郑州大学附属肿瘤医院肝胆胰外科

出　　处：《河南外科学杂志》2019年第5期9-11,共3页Henan Journal of Surgery

摘　　要：目的探讨肢体缺血预处理对大鼠肝脏缺血再灌注损伤的保护作用。方法将32只Wistar大鼠随机分为假手术组(SO)、肝脏缺血再灌注组(IR)、肝脏缺血预处理组(IPC+IR)和肢体缺血预处理组(LIPC+IR)。观察术后各组大鼠血液中炎症因子(IL-6,TNF-α)及氧化应激水平的差异;同时观察各组大鼠术后生存率及肝脏酶学水平的差异。结果LIPC组及IPC组大鼠术后血清AST、ALT均较IR组明显降低,术后第7天存活率较IR组明显提高,术后血清TNF-α、IL-6均较IR组明显降低,差异均有统计学意义(P<0.05)。LIPC组与IPC组比较无统计学意义(P>0.05)。LICP及ICP组大鼠术后体内MDA水平均较IR组降低,SOD水平均较IR组显著升高,差异均有统计学意义(P<0.05)。结论肢体缺血预处理能减轻大鼠肝脏缺血再灌注损伤,可能与减轻肝脏氧化应激水平有关。Objective To investigate the effects of limb ischemic preconditioning on hepatic ischemia reperfusion injury in rats.Methods Totally 32 Wistar rats were randomLy divided into sham operation(SO)group,liver ischemic reperfusion(IR)group,liver ischemic reperfusion preconditioning group(IPC+IR)and limb ischemia preconditioning group(RIPC+IR).The serum levels of inflammatory cytokines(IL-6,TNF-α)and oxidative stress of each group were analyzed,and the differences in postoperative survival rate and serum enzyme level were observed.Results The serum levels of AST and ALT in RIPC group and IPC group were significantly lower than that of the IR group(P<0.05);In addition,the survival rate of rats in RIPC group and IPC group at 7 days after surgery was significantly improved compared with that of IR group(P<0.05).The serum levels of TNF-αand IL-6 in RIPC group and IPC group were significantly lower than that of IR group(P<0.05).There was no significant difference between RIPC group and IPC group(P>0.05).The serum level of MDA in the RICP and ICP groups was lower than that of the IR group(P<0.05).On the contrary,the serum levels of SOD were significantly higher than that of IR group(P<0.05).Conclusion Limb ischemic preconditioning can alleviate hepatic ischemia reperfusion injury in rats,which may be related to the reduction of hepatic oxidative stress.

关 键 词：缺血预处理 缺血再灌注损伤 肝脏储备功能 肝切除 

分 类 号：R332[医药卫生—人体生理学]