基于巴戟天配方颗粒的标准汤剂特征图谱  

Characteristic map of Morinda officinalis How formula granules based on standard decoction

作　　者：李军山 李雪利 高杰 高晗 张肖建 孙福仁 牛丽颖 LI Junshan;LI Xueli;GAO Jie;GAO Han;ZHANG Xiaojian;SUN Furen;NIU Liying(Shineway Pharmaceutical Group Company Limited,Shijiazhuang,Hebei 051430,China;TCM Formula Granule Engineering Technology Research Center of Hebei,Shijiazhuang,Hebei 050200,China)

机构地区：[1]神威药业集团有限公司,河北石家庄051430 [2]河北省中药配方颗粒工程技术研究中心,河北石家庄050200

出　　处：《河北工业科技》2019年第5期362-368,共7页Hebei Journal of Industrial Science and Technology

基　　金：河北省科技计划项目(16272510);河北省重点研发计划项目(17272505D)

摘　　要：为了使巴戟天配方颗粒的质量标准更加科学规范,通过对15批产自不同地区的巴戟天饮片进行标准汤剂的制备,以及对标准汤剂干膏粉进行研究,确定了巴戟天标准汤剂的出膏率范围;采用高效液相色谱法对主含成分耐斯糖进行了含量测定,研究了巴戟天配方颗粒的HPLC特征图谱分析方法,并对特征谱图中的水晶兰苷进行了指认。结果表明,在以流动相作为提取溶剂、甲醇-水(二者体积比为3∶97)为流动相的条件下,耐斯糖含量的转移率范围为73.21%~94.49%,标准汤剂出膏率范围为49.05%~55.87%。基于巴戟天配方颗粒的标准汤剂特征图谱研究方法严谨、可行,更加科学地阐明了配方颗粒与标准汤剂研究的一致性。In order to make the quality standard of Morinda officinalis How prescription granules more scientific and standardized,15 batches of Morinda officinalis How decoction pieces produced in different regions are prepared with standard decoction,and the dry extract powder of standard decoction is studied to determine the range of extraction rate of Morinda officinalis standard decoction.The content of the main component Nystose is determined by high performance liquid chromatography.The HPLC characteristic chromatogram analysis method of Morinda officinalis How prescription granules is researched,and the crystal glycoside in the characteristic chromatogram is identified.The results show that under the flow phase as extraction solvent,methanol-water(3∶97) as mobile phase,the transfer rate of nesaccharide content is in the range of 73.21%~94.49%,and the extraction rate of standard decoction is in the range of 49.05%~55.87%.The quality standard established by the standard decoctio n characteristic map research institute based on Morinda officinalis How prescription granules is rigorous and feasible,which more scientifically illustrates the consistency between the prescription granules and the standard decoction research.

关 键 词：中药化学 巴戟天 标准汤剂 转移率 出膏率 特征图谱 

分 类 号：R284.1[医药卫生—中药学]