增生型糖尿病视网膜病变患者眼玻璃体、增生膜组织LncRNA ANRIL表达及意义  

Expression and significance of LncRNA ANRIL in vitreous and proliferative membranes of proliferative diabetic retinopathy patients

作　　者：陈日红 杨依玲 王宏飞 吴荣辉 CHEN Rihong;YANG Yiling;WANG Hongfei;WU Ronghui(Department of Ophtalmology,Zhejiang Sian International Hospital,Jiaxing,Zhejiang 314000,China)

机构地区：[1]浙江新安国际医院眼科

出　　处：《重庆医学》2019年第18期3138-3142,共5页Chongqing medicine

摘　　要：目的探讨增生型糖尿病视网膜病变(PDR)患者眼玻璃体、增生膜组织中长链非编码RNA ANRIL(LncRNA ANRIL)的表达及其意义。方法选取2017年6月至2018年6月该院收治的58例PDR患者为PDR组,35例特发性黄斑裂孔患者为对照组。实时定量聚合酶链反应(qRT-PCR)检测两组患者玻璃体、增生膜组织中LncRNA ANRIL、血管内皮生长因子(VEGF)mRNA表达;Western blot法检测增生膜组织中VEGF蛋白表达;采用Pearson法分析玻璃体、增生膜组织中LncRNA ANRIL与VEGF表达的相关性。结果PDR组患者玻璃体、增生膜组织中LncRNA ANRIL、VEGF mRNA相对表达水平均明显高于对照组(P=0.001);PDR组患者增生膜组织中VEGF蛋白相对表达水平明显高于对照组(P=0.001);PDR组患者玻璃体及增生膜组织中LncRN AANRIL与VEGF表达水平均呈正相关(P=0.001)。结论PDR患者玻璃体及增生膜组织中LncRNA ANRIL表达水平升高,其可能通过调控VEGF表达进而参与PDR发生过程。Objective To investigate the expression and significance of long non-coding RNA ANRIL (LncRNA ANRIL) in vitreous and proliferative membranes of proliferative diabetic retinopathy (PDR) patients. Methods A total of 58 patients with PDR admitted to this hospital from June 2017 to June 2018 were selected as the PDR group and 35 patients with idiopathic macular hole as the control group.Quantitative Real-time PCR (qRT-PCR) was used to detect the expressions of LncRNA ANRIL and vascular endothelial growth factor (VEGF) mRNA in vitreous and proliferative membranous of the two groups;the expression of VEGF protein was detected by Western blot.Pearson method was used to analyze the correlation between LncRNA ANRIL and the expression of VEGF in vitreous and proliferative membranous. Results The relative expressions of LncRNA ANRIL and VEGF mRNA in vitreous and proliferative membranous of the PDR group were significantly higher than those of the control group (P=0.001);the expression of VEGF protein in the PDR group was significantly higher than that in the control group (P=0.001);the expressions of LncRNA ANRIL in vitreous and proliferative membranous of the PDR group were positively correlated with VEGF (P=0.001). Conclusion The expression of LncRNA ANRIL in vitreous and proliferative membrane increased in patients with PDR,it may be involved in the process of PDR by regulating the expression of VEGF.

关 键 词：增生型糖尿病视网膜病变 玻璃体 增生膜 长链非编码RNAANRIL 

分 类 号：R587.2[医药卫生—内分泌]