3种环境因子对虎斑乌贼受精卵孵化的影响  

Effects of three environmental factors on hatching rate of fertilized eggs of Sepia pharaonis

作　　者：全成 莫金凤 周萌 黄培卫 黄国光 张海发 石和荣 甘松永 吴灶和 林蠡 QUAN Cheng;MO Jinfeng;ZHOU Meng;HUANG Peiwei;HUANG Guoguang;ZHANG Haifa;SHI Herong;GAN Songyong;WU Zaohe;LIN Li(Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangzhou 510225, China;College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;Guangdong Provincial Marine Fisheries Experimental Center, Huizhou 516081, China)

机构地区：[1]广州市水产病害与水禽养殖重点实验室,广东广州510225 [2]仲恺农业工程学院动物科技学院,广东广州510225 [3]广东省海洋渔业试验中心,广东惠州516081

出　　处：《仲恺农业工程学院学报》2019年第3期6-9,共4页Journal of Zhongkai University of Agriculture and Engineering

基　　金：广东省高等教育“创新强校工程”专项基金(KA170500G)资助项目

摘　　要：虎斑乌贼是一种在国内外都具有较高销量的重要的海洋渔业产品,其受精卵孵化率较低一直是限制其人工繁育的主要因素.本文对虎斑乌贼受精卵孵化的温度、盐度和pH值进行研究,探究其孵化的适宜生态条件,为虎斑乌贼的人工育苗提供基础数据.采用单因子试验,分别设置了不同温度(18、21、24、27、30和33℃)、盐度(15‰、18‰、21‰、24‰、27‰、30‰、33‰和36‰)和pH值(7.0、7.5、8.0和8.5),检测不同条件下虎斑乌贼受精卵孵化率和孵化时间.结果显示,受精卵可孵化温度为21~30℃;在27℃时,孵化率(72.2%)显著升高(P<0.05),随着温度的升高,孵化时间呈现缩短的趋势.在30℃时,孵化时间显著缩短(P<0.05).受精卵可孵化盐度为27‰~36‰;在最适盐度范围(30‰~33‰)下孵化率为70%~80%.pH值对虎斑乌贼受精卵孵化率影响不显著(P>0.05),在pH值7.0~8.5条件下均可孵化,孵化率均大于70%.因此,在温度24~27℃,盐度30‰~33‰和pH 7.0~8.5范围内,虎斑乌贼受精卵孵化较高.The cuttlefish Sepia pharaonis , an important fishery species in tropical archipelagic environments is highly required in both local and international market trades. Therefore, intensive efforts are currently undertaken to develop practical effects of fundamental physical and chemical parameters on the hatching rate of S. pharaonis fertilized eggs. In this report, the effects of three environmental factors that including temperature, salinity, and pH value were investigated, to understand the suitable ecological conditions for S. pharaonis hatching and enhancement of stocks to provide necessary data for the artificial breeding of S. pharaonis . The present study was conducted following a factorial design to evaluate the appropriate breeding parameters of cuttlefish at different temperatures (18, 21, 24, 27, 30 and 33 ℃), various salinity at (15‰, 18‰, 21‰, 24‰, 27‰, 30‰, 33‰ and 36‰) and at altered pH ranges (7.0, 7.5, 8.0 and 8.5). Results indicate that different temperatures had significant effects ( P <0.05) towards hatching of fertilized eggs at 21-30 ℃. With the increase of temperature, the incubation period was significantly shortened, and the optimal temperature was 27 ℃ with the hatching rate of 72.2%. The salinity has a significant effect on the hatching rate of S. pharaonis fertilized eggs at 27‰-36‰, and the optimum salinity ranged from 30‰-33 ‰ with the hatching rate of 70%-80%. By contrast, the effect of pH value on the hatching rate of S. pharaonis was not significant ( P >0.05), and the hatching rate was all greater than 70% under the pH values of 7.0-8.5. Regardless of pH, the temperature and salinity are critical factors affecting the hatchability performance of S. pharaonis fertilized eggs. Collectively, the present findings suggest that the artificially hatching conditions for the fertilized egg of S. pharaonis could be set as 24-27 ℃, 30‰-33‰ and pH value at 7.0-8.5 and these patterns may prove helpful in the maintenance of hatchery conditions for aquacultur

关 键 词：虎斑乌贼(Sepia pharaonis) 受精卵 环境因子 孵化 

分 类 号：S965[农业科学—水产养殖]