HPLC法测定霍山石斛中夏佛塔苷、异夏佛塔苷的含量  

Determination of Contents of Schaftoside and Isoschaftoside in Dendrobium huoshanense by HPLC

作　　者：杨丽娥[1] 叶家宏[2] 周楚娟 梁芷韵 黄月纯[1,2] 顺庆生[3] 魏刚[4] YANG Li-E;YE Jia-Hong;ZHOU Chu-Juan;LIANG Zhi-Yun;HUANG Yue-Chun;SHUN Qing-Sheng;WEI Gang(The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405 Guangdong,China;The FirstClinical Medical School of Guangzhou University of Chinese Medicine,Guangzhou 510006 Guangdong,China;ShanghaiAcademy of Health Sciences,Shanghai 200237,China;School of Pharmaceutical Sciences,Guangzhou Universityof Chinese Medicine,Guangzhou 510006 Guangdong,China)

机构地区：[1]广州中医药大学第一附属医院,广东广州510405 [2]广州中医药大学第一临床医学院,广东广州510006 [3]上海健康职业技术学院,上海200237 [4]广州中医药大学中药学院,广东广州510006

出　　处：《广州中医药大学学报》2019年第9期1431-1437,共7页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：【目的】建立霍山石斛中夏佛塔苷和异夏佛塔苷的含量测定方法。【方法】采用高效液相色谱(HPLC)法,ZorbaxSB-Aq色谱柱(250mm×4.6mm,5μm),流动相为乙腈(A)—体积分数为0.4%的甲酸溶液(B)(V∶V=15∶85),检测波长为340nm,流速为1mL·min-1,柱温为30℃。【结果】夏佛塔苷、异夏佛塔苷线性范围分别为5.84~70.08μg/mL、9.64~115.68μg/mL(r=0.9999)。夏佛塔苷的平均加样回收率为98.58%,相对标准偏差(RSD,sR)为1.82%;异夏佛塔苷的平均加样回收率为103.87%,sR为0.90%。平均加样回收率范围在99.76%~102.07%,sR均小于3.0%。11批样品夏佛塔苷和异夏佛塔苷的含量分别为0.0122~0.0866mg/g、0.0297~0.1463mg/g。【结论】该含量测定方法准确可靠,重复性好,可为霍山石斛的质量控制提供参考依据。Objective To establish a method for the determination of schaftoside and isoschaftoside in Dendrobium huoshanense.Methods High performance liquid chromatography(HPLC)was performed on Zorbax SB-Aq column(250 mm×4.6 mm,5μm),the mobile phase was acetonitrile-0.4%(volume fraction)formic acid solution(V∶V=15∶85),and the detection wavelength was 340 nm,flow rate was 1 mL·min-1,and column temperature was 30℃.Results The linear range of schaftoside and isoschaftoside was 5.84-70.08μg/mL and 9.64-115.68μg/mL(r=0.999 9),respectively.The average recovery of schaftoside was 98.58%,the relative standard deviation(RSD,sR)being 1.82%;the average recovery of isoschaftoside was 103.87%,sR being 0.90%.The range of average recovery was 99.76%-102.07%,and all sR was less than 3.0%.The contents of schaftoside and isoschaftoside in 11 batches of samples were 0.012 2-0.086 6 mg/g and 0.029 7-0.146 3 mg/g,respectively.Conclusion The determination method is accurate,reliable and reproducible,which can provide reference for quality control of Dendrobium huoshanense.

关 键 词：霍山石斛 夏佛塔苷 异夏佛塔苷 高效液相色谱法 质量控制 

分 类 号：R284.1[医药卫生—中药学]