Cryptosporidium parvum oocyst directed assembly of gold nanoparticles and graphene oxide  

作　　者：Sona Jain Zhicheng Huang Brent R. Dixon Syed Sattar Juewen Liu 

机构地区：[1]Department of Chemistry,Waterloo Institute for Nanotechnology,University of Waterloo,Ontario,N2L3G1,Canada [2]Bureau of Microbial Hazards,Food Directorate,Health Canada,Ontario,K1A 0K9,Canada [3]Faculty of Medicine,University of Ottawa,Ontario,K1H 8M5,Canada

出　　处：《Frontiers of Chemical Science and Engineering》2019年第3期608-615,F0004,共9页化学科学与工程前沿（英文版）

摘　　要：Understanding the interactions between inorganic nanomaterials and biological species is an important topic for surface and environmental chemistry. In this work, we systematically studied the oocysts of Cryptosporidium pa r v u m as a model protozoan parasite and its interaction with gold nanoparticles (AuNPs) and graphene oxide (GO). The as-prepared citrate-capped AuNPs adsorb strongly on the oocysts leading to a vivid color change. The adsorption of the AuNPs was confirmed by transmission electron microscopy. Heat treatment fully inhibited the color change, indicating a large change of surface chemistry of the oocysts that can be probed by the AuNPs. Adding proteases such as trypsin and proteinase K partially inhibited the color change. DNA-capped AuNPs, on the other hand, could not be adsorbed by the oocysts. GO was found to wrap around the oocysts forming a conformal shell reflecting the shape of the oocysts. Both citratecapped AuNPs and GO compromised the membrane integrity of the oocysts as indicated by the propidium iodide staining experiment, and they may be potentially used for inactivating the oocysts. This is the first example of using nanomaterials to probe the surface of the oocysts, and it suggests the possibility of using such organisms to template the assembly of nanomaterials.

关 键 词：NANOMATERIALS TOXICOLOGY WATER biosensors 

分 类 号：TQ[化学工程]