对血清碘的砷铈催化分光光度测定方法的验证  

Verification of a method for determination of iodine by As^3+-Ce^4+ catalytic spectrophotometry

作　　者：徐署东 赵立胜[1] 刘婷婷[1] 李卫东[1] Xu Shudong;Zhao Lisheng;Liu Tingting;Li Weidong(Department of Endemic and Parasitic Diseases,Anhui Center for Disease Control and Prevention,Hefei 230601,China)

机构地区：[1]安徽省疾病预防控制中心地方病与寄生虫病防治科,合肥230601

出　　处：《中华地方病学杂志》2019年第9期747-750,共4页Chinese Journal of Endemiology

摘　　要：目的验证卫生标准研制项目的血清碘的砷铈催化分光光度测定方法(简称本方法)的可行性及应用价值。方法收集成人血液样品,制备含0~300μg/L的碘标准曲线,参照《生物材料中化学物质的测定方法》,对本方法的线性关系、最低检测限、精密度和准确度进行实验验证,评价验证结果。结果在0~300μg/L标准曲线范围内,相关系数为-0.9997^-0.9994(n=6),最低检测限为5.13μg/L(取样量为100μl)。对3种不同碘浓度血清样进行重复测定,精密度验证显示,变异系数分别为0.89%、1.88%、0.67%;准确度验证显示,加碘标回收率为93.90%~107.04%,总平均回收率为99.48%。结论经验证,本方法应用于血清碘含量测定具有标准曲线线性关系好、精密度好、准确性高,符合生物样品分析的要求,适合推广应用。Objective To verify the feasibility and application value of arsenic-cerium catalytic spectrophotometry for determination of iodine in serum. Methods The blood samples of adults were collected and the iodine standard curve of 0 - 300 μg/L was prepared. Referring to "The Method for Determination of Chemical Substances in Biomaterials", the present method was tested in terms of linear relationship, minimum detection limit, precision and accuracy. Results The correlation coefficient of the method was - 0.999 7 -- 0.999 4 (n = 6) and the minimum detection limit was 5.13 μg/L in the range of 0 - 300 μg/L standard curve. Precision verification displayed that the variable coefficient of three different iodine concentration serum samples were 0.89%, 1.88%, 0.67%. And the accuracy verification displayed that the recovery of iodine standard was 93.90%- 107.04%, and the total average recovery was 99.48%. Conclusion The method has been proved to be of good linearity, high precision and high accuracy in the determination of serum iodine, which meets the requirements of biological sample analysis.

关 键 词：血清 碘 分光光度法 方法验证 

分 类 号：R599[医药卫生—内科学]