2017年深圳地区A/H3N2亚型流感病毒HA和NA基因的分子进化特征  

Molecular evolution of influenza A/H3N2 viruses HA and NA genes circulating in Shenzhen in 2017

作　　者：范颖 李雪云 房师松[2] 彭博[2] FAN Ying;LI Xue-yun;FANG Shi-song;PENG Bo(School of public health, Shenzhen Campus, Sun Yat-Sen University, Shenzhen 518107, China;Institute of pathogen biology, Center for Disease Control and Prevention of Shenzhen, Shenzhen 518055, China)

机构地区：[1]中山大学公共卫生学院深圳校区,深圳518107 [2]深圳市疾病预防控制中心病原生物研究所,深圳518055

出　　处：《中华疾病控制杂志》2019年第9期1114-1120,共7页Chinese Journal of Disease Control & Prevention

基　　金：深圳市科创委基础研究项目(JCYJ20170306160244540);深圳市卫生和计划生育委员会科研项目(SZFZ2017020)~~

摘　　要：目的了解深圳市2017年上半年流行的甲型H3N2亚型流感病毒HA和NA基因的分子进化特征,为预测流感病毒流行和变异提供科学依据。方法利用DNAStar、MEGA 7.0等生物信息学软件对研究分离的40株H3N2流感病毒的HA和NA基因及其编码的氨基酸序列进行比对,构建分子进化树,分析基因特征和变异情况。结果深圳分离株的同源性均达到97.8%~100.0%,位于亚洲北美人源分支。与世界卫生组织(World Health Organization, WHO)推荐的疫苗株A/Switzerland/9715293/2013(H3N2)和A/Hong Kong/4801/2014(H3N2)相比,有较高的序列相似性;与当年疫苗株对比发现,HA发生6个抗原位点和2个受体结合位点的改变,NA出现第151位酶活性位点的改变;HA和NA的潜在N-糖基化位点在数量和位置上也发生变化。结论深圳市2017年上半年甲型H3N2亚型流感病毒在流行过程中尚未发生明显变异,目前推荐的疫苗株仍对深圳地区人群有一定保护作用,HA和NA多个氨基酸位点的变异提示仍需加强H3N2亚型流感病毒分子水平的动态监测。Objective To investigate the molecular evolution of the Hemagglutinin(HA) and Neuraminidase(NA) genes of influenza A/H3 N2 viruses in Shenzhen in the first half of 2017, so as to provide scientific basis for predicating influenza epidemic and variation. Methods A total of 40 influenza A/H3 N2 viruses strains were selected and the molecular phylogenetic trees were constructed by bioinformatics software DNAStar, MEGA 7.0, etc. Then, the genetic characteristics and variation of HA and NA genes along with corresponding amino acids were analyzed. Results The homology of Shenzhen isolates reached 97.8%-100.0%, which located in the human-derived branch of Asia and North America. Compared with the vaccine strains A/Switzerland/9715293/2013(H3 N2) and A/Hong Kong 14801/2014(H3 N2) recommended by world Health Oraganication(WHO), there was a higher sequence similarity. Compared with the vaccine strain, HA and NA proteins had a number of amino acid sites replaced, of which HA 6 antigen sites and 2 receptor binding sites change;NA had a mutation of D151 N/G located in enzyme activity sites. Potential N-glycosylation sites for HA and NA also changed. Conclusions The influenza A/H3 N2 viruses in Shenzhen in the first half of 2017 has not yet formed a new subtype in the epidemic. Currently, the recommended vaccine strains still have some protective effects on the population. The replacement mutation of multiple amino acids sites of HA and NA suggests that the dynamic monitoring of molecular level of influenza A/H3 N2 viruses need to be strengthened.

关 键 词：H3N2亚型流感病毒 HA基因 NA基因 分子进化 序列分析 

分 类 号：R446.5[医药卫生—诊断学] R373.1[医药卫生—临床医学]