基于绿色低共熔溶剂法高效提取鸡骨草中的黄酮和皂苷  

Green and efficient extraction of flavoniods and saponins from Abrus cantoniensis Hance by deep eutectic solvents

作　　者：陈冉[1] 李德慧[1] 阮桂发 刘珊 孙科[1] 黄锁义[1] 欧阳熙林[1] CHEN Ran;LI De-hui;RUAN Gui-fa;LIU Shan;SUN Ke;HUANG Suo-yi;OUYANG Xi-lin(Youjiang Medical University for Nationalities,Baise 533000,China)

机构地区：[1]右江民族医学院

出　　处：《天然产物研究与开发》2019年第9期1632-1640,共9页Natural Product Research and Development

基　　金：右江民族医学院大学生创新创业训练项目(20171059 9114);广西自然科学基金(2017GXNSFAA198031)

摘　　要：为了研究鸡骨草中总黄酮和总皂苷的最佳提取工艺,通过单因素试验和星点设计-响应面法,对低共熔溶剂的性质(如种类、组分摩尔比、含水量)、提取温度、提取时间、液料比等多个因素进行考察。结果显示,总黄酮的最佳提取条件为:摩尔比1∶2、含水量30%的氯化胆碱/乙二醇作溶剂,提取温度80℃,提取时间40min,液料比15∶1(mL/g);总皂苷的最佳提取条件为∶摩尔比1∶4、含水量25%的氯化胆碱/乳酸作溶剂,提取温度80℃,提取时间64min,液料比56∶1(mL/g)。在最佳条件下,总黄酮和总皂苷的提取率较传统提取溶剂分别提高了33.3%和96.4%。本研究为鸡骨草中黄酮和皂苷的高效、安全提取提供了一条新思路。In order to research the optimal extraction process of total flavoniods and total saponins from Abrus cantoniensis Hance.Properties of DESs (including type,component mole ratio and water content),extraction temperature,extraction time,and liquid-solid ratio were studied by using single factor experiment and Box-Behnken experimental design combined with responsesurface methodology(BBD-RSM).The results indicated that the optimal extraction conditions for total flavoniods were as follows:ChCl/ethylene glycol with mole ratio of 1 ∶2 and water content of 30% as solvent,extraction temperature of 80 ℃,extraction time of 40 min,and liquid-solid ratio of 15 ∶1 (mL/g).The optimum conditions for total saponins were as follows:ChCl/lactic acid with mole ratio of 1 ∶4 and water content of 25% as solvent,extraction temperature of 80 ℃,extraction time of 64 min,liquid-solid ratio of 56 ∶1 (mL/g).Under the optimum conditions,the extraction yields of total flavoniods and total saponins increased by 33.3% and 96.4%,respectively,compared with conventional extraction solvents.The research could provide a new idea for efficient and safe extraction of flavoniods and saponins from Abrus cantoniensis Hance.

关 键 词：低共熔溶剂 鸡骨草 星点设计-响应面法 总黄酮 总皂苷 

分 类 号：R284.2[医药卫生—中药学]