荧光蛋白及其在微生物过程工程中的应用  

Fluorescent proteins and their applications in microbial strain engineering

作　　者：毛洪丽 刘雨 张建国[1] MAO Hongli;LIU Yu;ZHANG Jianguo(Institute of Food Science and Engineering,School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学医疗器械与食品学院食品科学与工程研究所

出　　处：《食品与发酵工业》2019年第17期252-257,共6页Food and Fermentation Industries

基　　金：国家自然科学基金项目(31870045、21306112)

摘　　要：荧光蛋白是一系列在紫外光激发下产生荧光的发光蛋白,以其荧光稳定性好、灵敏度高、无毒害等优点广泛应用于诸多领域。目前成功开发的荧光蛋白有绿色荧光蛋白(green fluorescent protein,GFP)、蓝色荧光蛋白(blue fluorescent protein,BFP)、青色荧光蛋白(cyan fluoroscent protein,CFP)、黄色荧光蛋白(yellow fluorescent protein,YFP)、红色荧光蛋白(red fluorescent protein,RFP)。荧光蛋白在微生物工程中菌株筛选方面的应用逐渐成熟,菌株筛选是菌种工程中较为耗时、费力的步骤,荧光蛋白为菌株筛选提供了新突破口。本文从荧光蛋白的基础研究出发,综述了近年来荧光蛋白在微生物工程中菌株筛选方面的进展和基于荧光蛋白的新型微生物筛选技术,为荧光蛋白等其他发光蛋白在微生物菌种工程的应用提供参考。Fluorescent proteins are a series of luminescent proteins under UV light. Fluorescent proteins are widely used as markers in many fields due to their good stability,high sensitivity and non-toxicity. Currently,successfully established fluorescent proteins include green fluorescent protein (GFP),blue fluorescent protein (BFP),cyan fluorescent protein (CFP),yellow fluorescent protein (YFP) and red fluorescent protein (RFP). Applications of fluorescent proteins in strain screening during microbial engineering have been well developed. Strain screening is time-consuming and labor-intensive,and fluorescent proteins provide a breakthrough for high-throughput screening. This review started with basic research work on fluorescent proteins and summarized the applications of fluorescent proteins in microbial strain engineering as well as new screening technologies. This review will provide a basis for applying fluorescent proteins and other luminescent proteins in microbial strain engineering.

关 键 词：荧光蛋白 菌种工程 筛选策略 微流体技术 

分 类 号：Q93[生物学—微生物学]