转录组测序研究兰州百合抗冻关键基因及途径  

Study on Key Anti-freeze Genes and Pathways of Lanzhou Lily(Lilium davidii var. unicolor) by Transcriptome Sequencing

作　　者：田雪慧[1,2] 郁继华 颉建明 TIAN Xuehui;YU Jihua;XIE Jianming(Department of Ecological and Environmental Engineering,Yangling Vocational Technical College,Yangling 712100,China;College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]杨凌职业技术学院生态环境工程分院,陕西杨凌712100 [2]甘肃农业大学园艺学院,甘肃兰州730070

出　　处：《广东农业科学》2019年第8期35-43,共9页Guangdong Agricultural Sciences

基　　金：国家重点研发计划项目(2016YFD0201005);杨凌职业技术学院自然科学基金(A2017027);杨凌示范区食用百合引种示范项目(TS-2016-06)

摘　　要：【目的】百合(Lilium)是国家卫生部首批公布的药食同源植物,也是著名的观赏植物。低温冻害严重影响植物的产量和分布,探究兰州百合对零下低温胁迫应答的分子机制。【方法】采用高通量IlluminaHiseq测序平台对兰州百合的20℃常温对照与-4℃寒冷处理(D)进行测序,并对测序数据进行分析研究。【结果】处理24h后检测到24465个差异表达的基因,筛选后获得4143个表达量上升的基因和4415个表达量下降的基因,占差异表达基因总数的10.27%。【结论】经富集分析认为,与蛋白激酶、蛋白磷酸酶、碳代谢以及ABA等相关的基因可作为研究兰州百合零下低温响应机制的主要研究对象。qRT-PCR分析表明,随机选出的10个差异性表达基因的表达趋势与高通量测序结果相一致。此外,吲哚-3-甘油磷酸合成酶、蛋白磷酸酶、已糖激酶、钙结合蛋白、叶绿素a/b结合蛋白等基因可作为与兰州百合寒冷响应相关的应答候选基因,为揭示兰州百合抗冻分子机制奠定了基础。【Objective】Lily(Lilium)is one of the first medical and edible plants published by the Ministry of Health,and is also a famous ornamental plant.As low temperature freezing damage seriously affecting the yield and distribution of plants,the study was to explore the molecular mechanism of the response of Lanzhou lily to subzero temperature stress【.Method】By using Illumina HiSeq transcriptome sequencing platform,sequencing was conducted to Lanzhou lily(L.davidii var.unicolor)exposed to two different temperature conditions,including,normal temperature treatment at 20℃(CK)and cold treatment at-4℃(D),and the sequencing data were analyzed.【Result】After being treated for 24 h,a total of 24 465 differentially expressed genes(DEGs)were identified,which included 4 143 significantly upregulated genes and 4 415 significantly downregulated genes,accounting for 10.27%of the total number of DEGs.【Conclusion】After enrichment analysis,DEGs related to protein kinase,protein phosphatase,carbon metabolism and ABA could be selected as main research objects for further studying the subzero cold response mechanism of Lanzhou lily.qRT-PCR analysis showed that the expression trend of 10 randomly selected DEGs coincided with the results of high-throughput sequencing.In addition,indol-3-glycerophosphate synthase,protein phosphatase,glycokinase,calcium-binding protein,and chlorophyll a/b binding protein were also selected as candidate genes that related to the anti-freeze responses of Lanzhou lily,Which lay a foundation for revealing the underlying mechanism of anti-freeze molecular of Lanzhou lily.

关 键 词：兰州百合 低温胁迫 转录组测序 差异基因 抗冻基因 通路分析 分子机制 

分 类 号：S682.29[农业科学—观赏园艺]