百合茎尖培养材料的筛选及其组培配方的优化  

Screening of Lily Shoot Tip Culture Materials and Optimization of Tissue Culture Formula

作　　者：吴青青 王维泽 崔嵬[1] 石乐娟[1] 李正丽 文林宏[1] WU Qingqing;WANG Weize;CUI Wei;SHI Lejuan;LI Zhengli;WEN Linhong(Institute of Horticulture,Guizhou Academy of AgriculturalSciences/Guizhou Horticultural Engineering Technology Research Center,Guiyang,Guizhou 550009,China)

机构地区：[1]贵州省农业科学院园艺研究所/贵州省园艺工程技术研究中心

出　　处：《贵州农业科学》2019年第9期69-73,共5页Guizhou Agricultural Sciences

基　　金：贵州省科技厅农业攻关项目“野百合优良种质的鉴选及配套栽培技术开发与应用”[黔科合NY字（2010）3088]，“百合OT×O远缘杂交及新品种选育”[黔科合NY(2015）3012-1];贵州省科技厅涉农科技支撑计划项目“食用百合脱毒快繁及产业化关键技术研究”[黔科合支撑（2017）2527];贵州省科技厅科技成果应用及产业化项目“百合自育新品种‘贵阳红’生产示范推广”[黔科合成转字（2014）5027]，“鲜切花百合产业科技成果示范与推广”[黔科合成转字（2015）5321-2];贵州省科技厅科技平台及人才团队建设项目“贵州省特色兰花、百合种质资源研究与应用科技创新人才团队”[黔科合平台人才（2016）5628]，“贵州省高层次创新型人才培养计划——‘百’层次人才”[黔科合平台人才（2016）5671];贵州省农业科学院科技成果培育与人才培养项目“百合脱毒技术研究”[黔农科院CR合字（2014）];贵州省农业科学院院地合作项目“百合优新品红引进、鉴选、繁育与生态栽培示范”[院地农科合字（2015）];贵州省农业科学院青年基金项目“石斛花在试管内发育过程中FT同源基因表达模式解析”[黔农科院青年基金（2017）08]

摘　　要：为掌握百合茎尖培养脱毒技术,为百合脱毒苗的大批量生产提供技术支撑,以百合品种卡瓦娜(Corvara)为材料,对不同来源的培养材料(未经处理的组培苗、经过4℃低温处理40d后的组培苗和商品球)进行茎尖观察,并对适合培养的茎尖材料进行不同配方的组织培养。结果表明:未经处理的组培苗其茎尖生长点未见叶原基,其分生组织不具备立刻发育成茎的能力,不适宜作茎尖培养材料;经过低温处理的组培苗部分茎尖为完整的芽,顶端生长点活动旺盛,适宜进行茎尖培养,部分则无叶原基等其他结构;商品球茎尖部位明显且生长旺盛,最适宜作茎尖培养材料。百合茎尖组织培养的最佳培养基配方为MS+6-BA1.0mg/L+NAA0.5mg/L+蔗糖30g/L+琼脂7g/L+活性炭1g/L,pH5.8。To master the detoxification technology of lily shoot tip culture,and to provide technical support for mass production of virus-free lily seedlings,using Corvara as the material,the shoot tips of different sources(untreated tissue culture seedlings,tissue culture seedlings and commercial bulbs treated at 4℃for 40 days)were observed.Meanwhile,the stem tip materials suitable for culture were cultured with different formulations.Results:The shoot tip growth point of untreated tissue culture seedlings did not have leaf primordia,and its meristem did not have the ability to develop into stem immediately,so it was not suitable to be used as stem tip culture material;Nevertheless,some of the shoot tips of tissue culture seedlings treated with low temperature were complete buds,and the apical growing point was active,which was suitable for shoot tip culture,and some of them had no leaf primordia and other structures;Meanwhile,commercial bulb tip was obvious with vigorous growth,so it was the most suitable material for stem tip culture.The optimum medium for shoot tip tissue culture of lily was MS+6-BA 1.0 mg/L+NAA0.5 mg/L+sucrose 30 g/L+agar 7 g/L+activated carbon 1 g/L,pH 5.8.

关 键 词：百合 茎尖 脱毒 组织培养 

分 类 号：S682.29[农业科学—观赏园艺]