氢氧化细菌SDW-16的分离鉴定及促生特性  

Isolation, Identification and Growth-Promoting Properties of Hydrogen Oxidizing Bacterium SDW-16

作　　者：王琳 李志英[2] 常小娟 刘欣[2] 王卫卫[2] WANG Lin;LI Zhi-ying;CHANG Xiao-juan;LIU Xin;WANG Wei-wei(Dept. of Med. Technol.,Xi'an Med. Uni.,Xi'an 710021;Key Lab. of Res. Biol.& Biotech. in W. China,Minist. of Educ. NW Uni. Xi'an 710069)

机构地区：[1]西安医学院医学技术系,陕西西安710021 [2]西部生物资源与现代生物技术教育部重点实验室,陕西西安710069

出　　处：《微生物学杂志》2019年第4期40-45,共6页Journal of Microbiology

基　　金：国家自然科学基金项目(41571243);西安医学院配套基金项目(2017PT14);西安医学院国家基金培育项目(2017GJFY16)

摘　　要：利用气体循环培养体系从沙打旺根际土壤分离得到1株氢氧化细菌SDW-16(GenBank登录号:KF835389),16S rDNA序列分析和生理生化特征鉴定其为荧光假单胞菌(Pseudomonas fluorescens)。菌株对植物促生机制的初步研究表明菌株SDW-16除具有铁载体分泌能力外,还具有产IAA和ACC脱氨酶活性,其中产IAA量为(21.62±0.30)μg/mL,ACC脱氨酶活力高达(8 372.17±805.43) nmol/(mg·h)。菌株SDW-16具有多项促生能力且均高于其他菌株,说明菌株SDW-16有较高的促生特性,同时也初步证明了氢氧化细菌的促生机制。A hydrogen oxidizing bacterium strain SDW-16(GenBank accession number: KF835389) was isolated from the rhizosphere soil of Astragalus adsurgens Pall using the gas circulation culture system. The bacterium SDW-16 was identified as Pseudomonas fluorescens by 16 S rDNA sequence analysis and physiological and biochemical characteristics. Preliminary studies on the plant growth-promoting mechanism showed that the strain SDW-16 had the ability to produce IAA and ACC deaminase in addition to the iron carrier secretion capacity, and the production of IAA was(21.62±0.30)μg/mL, ACC deaminase activity was up to(8 372.17±805.43) nmol/(mg·h). The strain SDW-16 had a number of growth-promoting abilities and was higher than other strains, indicating that the strain SDW-16 had higher growth-promoting property, and also preliminarily have proved the promoting mechanism of the hydrogen-oxidizing bacteria.

关 键 词：氢氧化细菌 铁载体 IAA ACC脱氨酶 16S RDNA 

分 类 号：Q93-331[生物学—微生物学]