CVB对OL细胞和OL/BDV细胞I型IFN信号转导途径的影响  

Effect on Type I IFN Signal Transduction Path in OL and OL/BDV Cells Infected with CVB

作　　者：翟爱霞[1] 颜冬梅[2] 王海萍[2] 卜桐 崔乐乐 考文萍[1] 张凤民[1] ZHAI Ai-xia;YAN Dong-mei;WANG Hai-ping;BU Tong;CUI Le-le;KAO Wen-ping;ZHANG Feng-min(Teach.& Res. Div. of Microbiol.,Wu Lien-Teh Inst.,Key Lab. of Infect.& Immunol. of Heilongjiang Prov.,Pathobiol. Key Lab. of Heilongjiang Prov.,Sci.& Tech. Inno. Team for Infect.& Immunol. of Heilongjiang Prov.,Harbin 150081;4th AffiL Hosp.,Harbin Med. Uni.,Harbin 150001)

机构地区：[1]哈尔滨医科大学微生物学教研室伍连徳研究所黑龙江省感染与免疫重点实验室黑龙江省普通高校病原生物学重点实验室黑龙江省普通高校感染与免疫科技创新团队,黑龙江哈尔滨150081 [2]哈尔滨医科大学附属第四医院,黑龙江哈尔滨150001

出　　处：《微生物学杂志》2019年第4期46-51,共6页Journal of Microbiology

基　　金：黑龙江省教育厅科学技术研究项目(12541365);国家自然科学基金项目(81501737)

摘　　要：探讨柯萨奇病毒(coxsackievirus, CVB)对人类少突胶质细胞(oligodendrocyte, OL)和博尔纳病病毒(Borna disease virus, BDV)持续感染的OL细胞(OL/BDV)中I型干扰素(interferon, IFN)、microRNA-155(miR-155)表达以及干扰素调节因子(interferon regulatory factor, IRF)7细胞定位的影响。CVB感染OL细胞和OL/BDV细胞0、2、4、6、12和24 h,qPCR检测I型IFN mRNA和miR-155表达水平。OL细胞和OL/BDV细胞分别转染IRF7-EGFP质粒,CVB感染4 h,观察IRF7细胞定位情况。CVB感染OL细胞2、4、12和24 h,IFN-αmRNA和miR-155表达水平显著增加,IFN-βmRNA表达水平在4、12和24 h显著增加;CVB感染OL/BDV细胞IFN-α和IFN-βmRNA表达水平除0 h均显著增加,但miR-155表达仅在12和24 h显著增加。CVB感染OL细胞和OL/BDV细胞4 h,IRF7绿色荧光蛋白由细胞浆转移至细胞核内。综上所述,CVB可诱导OL细胞和OL/BDV细胞I型IFN、miR-155的表达,促进IRF7入核,从而激活I型IFN信号转导途径。Effects of coxsackievirus(CVB) on the expression of type I interferon(IFN) and microRNA-155(miR-155) as well as the localization of interferon regulatory factor(IRF) 7 in human oligodendrocyte(OL) and Borna disease virus(BDV) infected OL cells(OL/BDV) were investigated. After the CVB infection, the expression of type I IFN mRNA and miR-155 in OL cells and OL/BDV cells were detected by qPCR on 0, 2, 4, 6, 12 and 24 h and observed the localization of IRF7 cells. OL cells and OL/BDV cells were respectively transfected to IRF7-EGFP plasmid and infected with CVB for 4 h and observed the cell localization. The expression of IFN-α mRNA and miR-155 significantly increased at 2, 4, 12 and 24 h, and IFN-β mRNA significantly increased at 4, 12 and 24 h in OL cells. The expression of IFN-α and IFN-β mRNA in OL/BDV cells infected with CVB all increased significantly at most time points detected except 0 h, however, miR-155 increased only at 12 and 24 h. In OL cells and OL/BDV cells infected with CVB for 4 h, IRF7 green fluorescent protein was translocated from cytoplasm into nucleus. In summary of all above, CVB could induce the expression of type I IFN and miR-155 and promote the entrance of IRF7 into nucleus, thus activated the type I IFN signal transduction pathway.

关 键 词：柯萨奇病毒 OL细胞 OL/BDV细胞 I型IFN信号转导途径 microRNA-155 

分 类 号：Q939.93[生物学—微生物学]