耐碳青霉烯类鲍曼不动杆菌同源性及生物膜形成能力分析  

Homology and Biofilm-Forming Ability of Carbapenem Resistant Acinetobacter baumannii

作　　者：赵永鑫 王晓红[1] 王英[1] 郭宇航[1] 范学财[2] 王勇[1] 包名家[3] 张晓丽[1] ZHAO Yong-xin;WANG Xiao-hong;WANG Ying;GUO Yu-hang;FAN Xue-cai;WANG Yong;BAO Ming-jia;ZHANG Xiao-li(Dept. of Lab,1st.Affil. Hosp.,Jiamusi Uni.,Jiamusi 154007;Dept. of Lab.,2nd Affil. Hosp.,Jiamusi Uni.,Jiamusi 154003;CDC.,Jiamusi 154000)

机构地区：[1]佳木斯大学附属第一医院检验科,黑龙江佳木斯154007 [2]佳木斯大学附属第二医院检验科,黑龙江佳木斯154003 [3]佳木斯市疾病预防控制中心,黑龙江佳木斯154000

出　　处：《微生物学杂志》2019年第4期65-70,共6页Journal of Microbiology

基　　金：黑龙江省自然科学基金项目(D201224);黑龙江省卫生计生委科研项目(2017-405)

摘　　要：分析耐碳青霉烯类鲍曼不动杆菌(Carbapenem Resistant Acinetobacter baumannii,CRAB)的同源性,测定其生物膜形成能力,分析blaOXA基因携带与生物膜形成能力的相关性,探讨CRAB在医院暴发的影响因素。收集佳木斯大学附属第一医院非重复CRAB共35株,全自动微生物鉴定系统VITEK-II及体外敏感实验筛选CRAB,多序列位点分型(MLST)、肠道细菌基因重复序列聚合酶链式反应(ERIC-PCR)分析其同源性,结晶紫染色法定量检测生物膜形成能力。33株CRAB为ST 2型,1株ST 671型,1株ST 1199型为新发现的MLST分型。ERIC可分为A、B、C、D四型,A型9株,B型24株,C型1株,D型1株。CRAB及碳青霉烯类敏感鲍曼不动杆菌(CSAB)组生物膜形成能力差异有统计学意义(0.330±0.258、0.534±0.402,P<0.05);blaOXA-23、blaOXA-51、blaOXA-24基因携带与CRAB生物膜形成能力无明确相关性(P>0.05)。生物膜形成能力与碳青霉烯类耐药性的获得呈现负相关。ERIC-PCR分辨率较强,且与MLST具有一定相关性。ST2型为主要流行菌株,患者的高危因素以及医护人员的消毒意识可能与CRAB的暴发有关。The homology of carbapenem-resistant Acinetobacter baumannii(CRAB) was analyzed, its biofilm formation ability was tested, the reciprocity of the gene carrying of blaOXA and biofilm formation was analyzed, and the effective factors of the outbreak of CRAB in the hospital were investigated. A total of 35 non-repeated CRAB strains was collected in the first affiliated hospital of Jiamusi University, and screened the CRAB strains with full-automatic microorganism identification system VITEK-II as well as with in vitro sensitive experiment, multilocus sequence typing(MLST), enterobacterial repetitive intergenic consensus-polymerase chain reaction(ERIC-PCR) to analyze their homology, and crystal violet staining to quatitatively check their biofilm formation ability. In the hospital where the authors work, 33 strains of CRAB were ST type 2, one strain ST 671, and one strain ST 1199 was newly discovered MLST subtype. According to ERIC they could be divided into A, B, C, and D types, A type 9 strains, B type 24 strains, C type one strain, and D type one strain. And there was significant statistic differences of the biofilm formation ability of CRAB and carbapenem-sensitive Acinetobacter baumannii(CSAB) groups(0.330±0.258, 0.534±0.402, P<0.05);and there was no significant reciprocity between gene carrying of blaOXA-23, blaOXA-51, and blaOXA-24 gene and CRAB biofilm formation ability(P>0.05). And the biofilm formation ability was negatively correlated with carbapenem resistance obtainment. The resolution of ERIC-PCR was relatively strong, and it had a certain correlation with MLST. ST2 type was the main epidemic strain in the hospital. The high risk factors of the patients and the sterilization awareness of the doctors and nurses may be related to the outbreak of CRAB.

关 键 词：碳青霉烯类耐药 生物膜 同源性分析 

分 类 号：Q939.93[生物学—微生物学] R378.991[医药卫生—病原生物学]