PECAM-1和VCAM-1在侵入性胎盘患者血清及胎盘组织中的表达及意义  

Expression and significance of PECAM-1 and VCAM-1 in serum and placenta of invasive placenta patients

作　　者：宋学薇 刘亚梅 孙佳 胡鑫 纪江海 SONG Xuewei;LIU Yamei;SUN Jia;HU Xin;JI Jianghai(Department of Obstetrics and Gynecology,Cangzhou People's Hospital,Hebei Province,Cangzhou 061000,China)

机构地区：[1]河北省沧州市人民医院妇产科

出　　处：《疑难病杂志》2019年第9期936-939,950,共5页Chinese Journal of Difficult and Complicated Cases

基　　金：沧州市重点研发计划指导项目(172302029)

摘　　要：目的探讨血小板内皮细胞黏附分子-1(PECAM-1)和血管细胞黏附分子-1(VCAM-1)在侵入性胎盘患者血清及胎盘组织中的表达及其意义。方法选取2009年2月—2016年12月沧州市人民医院妇产科单胎晚期妊娠分娩的产妇共276例为研究对象,根据有无侵入性胎盘组织分为正常胎盘组138例、侵入性胎盘组138例;实时荧光逆转录PCR法、免疫组织化学法、酶联免疫吸附法测定2组产妇血清及胎盘组织中PECAM-1、VCAM-1mRNA及蛋白表达水平。结果侵入性胎盘组胎盘组织中PECAM-1、VCAM-1mRNA表达水平及蛋白表达评分高于正常胎盘组(t=43.685、64.578、77.448、112.108,P<0.01),侵入性胎盘组PECAM-1、VCAM-1蛋白表达阳性率高于正常胎盘组(χ^2=49.125、112.824,P<0.01);侵入性胎盘组血清PECAM-1、VCAM-1mRNA表达水平高于正常胎盘组(t=107.025、59.866,P<0.01),血清PECAM-1、VCAM-1蛋白表达水平高于正常胎盘组(t=110.430、70.550,P<0.01)。结论侵入性胎盘患者血清及胎盘组织中PECAM-1和VCAM-1mRNA、蛋白表达水平均升高,导致滋养细胞过度增殖,螺旋动脉血管损伤以及胎盘炎性反应,这与侵入性胎盘发病机制密切相关。Objective To investigate the expression and significance of platelet endothelial cell adhesion molecule-1(PECAM-1)and vascular cell adhesion molecule-1(VCAM-1)in serum and placenta of invasive placenta patients.Methods A total of 276 parturients of single late pregnancy in Cangzhou People's Hospital from February 2009 to December 2016 were selected as the study subjects.According to the presence or absence of invasive placenta,138 cases were divided into normal placenta group and invasive placenta group.Real-time fluorescent reverse transcription polymerase chain reaction,immunohistochemistry and enzyme-linked immunosorbent assay were used to detect the two groups.The expression of PECAM-1 and VCAM-1 in maternal serum and placenta.Results The expression levels of PECAM-1,VCAM-1 mRNA and protein in the placenta of the invasive placenta group were higher than those in the normal placenta group(t=43.685,t=64.578,t=77.448,t=112.108,P<0.01).The positive rate of PECAM-1 and VCAM-1 protein expression in the invasive placenta group was higher than that in the normal placenta group(χ^2=49.125,χ^2=112.824,P<0.01).The expression levels of serum PECAM-1 and VCAM-1 mRNA in the invasive placenta group were higher than those in the normal placenta group.The group(t=107.025,t=59.866,P<0.01),serum PECAM-1,VCAM-1 protein expression level was higher than the normal placenta group(t=110.430,t=70.550,P<0.01).Conclusion The expression of PECAM-1 and VCAM-1 in serum and placenta tissues of invasive placenta patients increased,which led to trophoblastic over-proliferation,spiral artery vascular injury and placental inflammatory response,which were closely related to the pathogenesis of invasive placenta.

关 键 词：血小板内皮细胞黏附分子-1 血管细胞黏附分子-1 侵入性胎盘 表达 意义 

分 类 号：R714.2[医药卫生—妇产科学]