拟轮枝镰孢菌毒素粗提液对玉米种子发芽及幼苗生长的影响  

Effect of the Crude Toxin of Fusarium verticillioides on Seed Germination and Seedling Growth in Maize

作　　者：刘春元[1] 邢小萍[1] 韩卫丽[1] LIU Chun-yuan;XING Xiao-ping;HAN Wei-li(College of Plant Protection,Henan Agricultural University,Zhengzhou,Henan 450002)

机构地区：[1]河南农业大学植物保护学院

出　　处：《安徽农业科学》2019年第19期158-161,192,共5页Journal of Anhui Agricultural Sciences

基　　金：国家重点研发计划项目(2017YFD0201700)

摘　　要：为建立玉米杂交种和自交系利用拟轮枝镰孢菌毒素抗性筛选的试验方法,制备拟轮枝镰孢菌毒素粗提液,测定不同稀释倍数毒素粗提液对2个玉米杂交种和4个玉米自交系处理4和6d后的种子发芽抑制率、胚根生长抑制率以及幼苗致萎效果,分析不同抗性玉米种质、毒素稀释倍数和处理时间对玉米种子发芽、胚根和幼苗生长的影响。结果表明,易感杂交种豫玉22与抗性杂交种农大108的种子发芽抑制率和胚根生长抑制率均存在极显著差异(P<0.01);在处理后4、6d时,前者的种子发芽抑制率、胚根生长抑制率分别比后者高7.2%、11.3%、12.7%、11.8%。在对玉米杂交种幼苗致萎蔫试验中,用稀释2倍和5倍的毒素粗提液处理,豫玉22与农大108杂交种幼苗间的萎蔫程度也存在显著差异(P<0.05)。拟轮枝镰孢菌毒素粗提液对不同抗性玉米种质种子发芽、幼苗生长的影响存在显著差异,为利用拟轮枝镰孢菌毒素粗提液对玉米品种进行苗枯病抗性鉴定和筛选提供理论依据。In order to establish a simple method for evaluating the resistance of maize hybrids and inbred lines,the crude toxin extract from the culture medium of Fusarium verticillioides was prepared,and the seed germination inhibition rate,radicle growth inhibition rate,as well as the wilting degree of 2 maize hybrids and 4 inbred lines under the treatment of the crude toxin at various dilutions and different treatment duration(4 or 6 d)were determined.The results showed that the susceptible hybrid Yuyu22 and resistant hybrid Nongda108 significantly differed in the inhibition rates of seed germination and radicle growth(P<0.01),and the inhibition rates of seed germination and radicle growth of Yuyu22 were 7.2%,11.3%and 12.7%,11.8%higher than those of Nongda 108.An obvious difference between Yuyu22 and Nongda108 was also found under the treatment of the crude toxin extract at 2 and 5 diluted solution(P<0.05).The effects of crude extracts of fusarium rotatum toxin on seed germination and seedling growth of different resistant maize germplasms were obviously different,which provided certain basis for identification and screening of resistance to bacterial blight of maize varieties by using crude extracts of fusarium rotatum toxin.

关 键 词：玉米苗枯病 拟轮枝镰孢菌 毒素粗提液 发芽率 胚根生长 

分 类 号：S432.1[农业科学—植物病理学]