西红花苷通过TGF-β1和NF-κB通路抑制草酸引起的肾小管细胞上皮间质转化  

Crocin inhibits oxalate-induced epithelial-mesenchymal transition of renal tubular epithelial cells via TGF-β1 and NF-κB signaling pathways

作　　者：刘亚东[1] 于时良[1] 刘健男[1] 安瑞华[1] LIU Ya-dong;YU Shi bang;LIU Jian-nan;AN Rui-hua(Department of Urology,First Affiliated Hospital of Harbin Medical University,Harbin 150001,China)

机构地区：[1]哈尔滨医科大学附属第一医院泌尿外科

出　　处：《现代泌尿外科杂志》2019年第9期754-757,共4页Journal of Modern Urology

基　　金：国家自然科学基金(No.81370803-2013)

摘　　要：目的研究西红花苷是否能通过转化生长因子β1(TGF-β1)和核因子-κB(NF-κB)通路抑制草酸引起的肾小管细胞上皮间质转化(EMT)的发生。方法MDCK培养后分为3组(空白对照组、草酸组、草酸+西红花苷组)分别予以相应干预。运用Western blot蛋白印迹分析检测E-钙黏附蛋白(E-cadherin)、神经-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)、TGF-β1、NF-κB的表达。分别检测不同组细胞中丙二醛(MDA)、超氧化物歧化酶(SOD)、活性氧(ROS)的表达。结果草酸会使TGF-β1、Ncadherin、Vimentin、NF-κB表达量增加,使E-cadherin表达下降。西红花苷会降低草酸引起的细胞ROS的增加。结论西红花苷通过TGF-β1和NF-κB通路抑制上皮间质转化。Objective To investigate whether crocin can inhibit oxalate-induced epithelial-mesenchymal transition(EMT)of renal tubular epithelial cells via TGF-β1 and NF-kB signaling pathways.Methods MDCK cells were divided into three groups:control group,oxalate group and crocin+oxalate group.The protein expressions of E-cadherin,N-cadherin,Vimentin,transforming growth factorβ1(TGF-β1)and nuclear factor-κB(NF-κB)were detected with Western blot.The levels of malondialdehyde(MDA),superoxide dismutase(SOD)and reactive oxygen species(ROS)were determined.Results Oxalate treatment increased the levels of TGF-β1,N-cadherin,Vimentin and NF-κB while decreased the expression of E-cadherin.The addition of crocin markedly reduced the level of ROS.Conclusion Crocin can inhibit oxalate-induced EMT of renal tubular epithelial cells through TGF-β1 and NF-κB pathways.

关 键 词：西红花苷 TGF-Β1 NF-ΚB 上皮间质转化(EMT) 活性氧(ROS) 草酸 

分 类 号：R692.4[医药卫生—泌尿科学]