基于碘刻蚀金银纳米立方块利用暗场显微镜高灵敏检测脑透析液中碘  

Sensitive Detection of Iodine in Rat Brain Dialysate by Dark Field Microscopy Based on Iodine Etching Au@Ag Nanocubes

作　　者：刘佳 李巧君 王超 李凯[1] 林雨青[1] LIU Jia;LI Qiao-Jun;WANG Chao;LI Kai;LIN Yu-Qing(Department of Chemistry,Capital Normal University,Beijing 100048,China)

机构地区：[1]首都师范大学化学系

出　　处：《分析化学》2019年第10期1695-1702,共8页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21575090);“十三五”时期北京市高校高层次教师队伍建设项目(No.CIT&TCD20190330);北京市教委科研项目(No.KM201810028008);首都师范大学青年创新研究团队与科技创新基础科研基金能力建设项目(Nos.19530050179,025185305000/195)资助~~

摘　　要：采用局域表面等离子体共振(LSPR)技术研究了金银纳米立方块(Au@Ag NCs)与I2的反应过程,以Au@Ag NCs为探针,在暗场显微镜(DFM)下分析探针与不同浓度I-/I2作用后的颜色比和散射光强度,发现探针颜色由蓝色逐渐变为黄绿色,同时其散射光强度不断下降。此外,通过透射电镜(TEM)和紫外-可见(UV-Vis)吸收光谱发现,Au@Ag NCs从边缘不断被氧化刻蚀,逐渐变成球形,最终变成类球形。以Au@Ag NCs作为探针检测I2,利用DFM检测I2的线性范围为0.1~50μmol/L,检出限为17 nmol/L,并将本方法应用于鼠脑透析液中I2的加标检测,探究了Au@Ag NCs与I-/I2之间的氧化还原反应过程。本研究为鼠脑透析液中I2的加标回收提供了一种新的高灵敏方法。The reaction process between Ag and I2 was investigated by the localized surface plasmon resonance(LSPR)technique.Au@Ag nanocubes(Au@Ag NCs)were used as a probe to analyze the different color ratio and scattering intensity of the probe and I2 after adding different concentrations of I-/I2 under the dark-field microscopy(DFM).Furthermore,transmission electron microscopy(TEM)and UV-Visible absorption spectra showed that Au@Ag NCs were continuously oxidized from the edges,gradually became spherical,and finally quasi-spherical.Finally,Au@Ag NCs were used as probes to test I2.The detection range was from 0.1μmol/L to 50μmol/L with a detection limit of 17 nmol/L.This work laid the foundation for the study of oxidative corrosion between Ag and I2,and also provided a new highly sensitive method for detection of I2 in brain rat dialysate.

关 键 词：金银纳米立方块 暗场显微镜 散射强度 碘 

分 类 号：O652[理学—分析化学] R446.1[理学—化学]