GATA4/5/6基因克隆及其在出生后鸭心脏和肝脏中的发育性表达模式  

Cloning of GATA4/5/6 Genes and Their Expression Patterns in Duck Hearts and Livers during Postnatal Development

作　　者：欧阳清渊 王郁石 胡深强[1] 李亮[1] 刘贺贺[1] 何桦[1] 王继文[1] OUYANG Qingyuan;WANG Yushi;HU Shenqiang;LI Liang;LIU Hehe;HE Hua;WANG Jiwen(College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130,China)

机构地区：[1]四川农业大学动物科技学院

出　　处：《畜牧兽医学报》2019年第9期1577-1588,共12页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家大学生创新性试验计划(201610626029);四川省畜禽育种攻关项目(2016NYZ0044)

摘　　要：旨在克隆和分析鸭内脏器官发育调控关键基因GATA4/5/6编码区,并初步揭示其mRNA表达量与出生后鸭心脏和肝脏发育的关系。本研究对鸭GATA4/5/6基因进行PCR扩增和克隆,对得到的编码区进行序列分析,采用qRT-PCR检测它们在0、2、4、6、8周龄鸭心脏和肝脏中的表达量,并对表达量与心脏和肝脏重量及器官指数进行相关分析。结果,获得鸭GATA4/5/6基因完整编码区序列,长度依次为1 242、1 182、1 173 bp。鸭GATA4/5/6均存在位于N端和C端的2个锌指结构域以及1个GATA-N转录激活结构域,3个基因间GATA-N转录激活域的氨基酸一致率低,而2个锌指结构在GATA4/5/6中共有区域的保守性很高,但GATA5的C端锌指结构和GATA6的N端锌指结构分别缺失了11和15个氨基酸。qRT-PCR结果显示,GATA4/5/6均在0~8周龄鸭心脏和肝脏中一直维持较高表达水平,但不同基因间的发育性表达模式明显不同,提示可能存在功能差异。进一步的相关性分析表明,鸭心脏和肝脏的重量与GATA4表达量极显著相关(P<0.01)。综上,本试验初步揭示GATA4/5/6在出生后鸭心脏和肝脏中持续性表达并具有各自特异的表达模式,并且可能由于GATA4具有更完整的锌指结构域使得其与心脏和肝脏发育关系尤为密切。The aim of this study was to clone and analyze the complete coding region sequences of GATA4/5/6, the key genes regulating the development of duck internal organs. The relationship between mRNA expression levels of GATA4/5/6 at 0, 2, 4, 6,8 weeks old and the development of heart and liver of duck was preliminarily revealed in duck. PCR amplification and cloning were performed on the GATA4/5/6 genes of ducks, and sequence analysis was performed on the coding regions. qRT-PCR was used to detect GATA4/5/6 expression in heart and liver of ducks at 0, 2, 4, 6, 8 weeks old. The correlationship analysis between expression level of GATA4/5/6 and heart and liver weight and organ index was performed. The complete coding region sequences of duck GATA4/5/6 genes were obtained by amplification, with the length of 1 242, 1 182 and 1 173 bp, respectively. Further analysis showed that GATA4/5/6 of ducks had 2 zinc finger domains located at the N-terminal and C-terminal and one GATA-N domain. The amino acid consistency rate of GATA-N transcriptional activation domain was low among the 3 genes. The common areas of the 2 zinc finger structures in GATA4/5/6 were higher, but the zinc finger structure at the C-terminal of GATA5 and the zinc finger structure at the N-terminal of GATA6 were the sequence deleting 11 and 15 amino acids, respectively. qRT-PCR results showed that GATA4/5/6 maintained a high expression level in the heart and liver of 0-8 weeks old ducks. But, the developmental expression patterns between different genes and tissues were significantly different. This phenomenon indicated that GATA4/5/6 might have different functions. Further correlation analysis showed that the weight of duck heart and liver was significantly correlated with GATA4 expression level(P<0.01). In conclusion, the present study preliminarily revealed that the sustained and specific expression of GATA4/5/6 might be related to the development of heart and liver in postnatal ducks. Moreover, GATA4 might be particularly closely related to the developme

关 键 词：鸭 肝脏 心脏 GATA4/5/6 基因克隆 发育性表达 相关分析 

分 类 号：S834.2[农业科学—畜牧学]