不同LED光质对两种黄花高山杜鹃组培增殖和生根的影响  

Effects of Different LED Light Quality on the Tissue Culture Proliferation and Rooting of Two Genotypes of Rhododendron

作　　者：廖多思 彭绿春[2,3,4,5] 林开文 解玮佳 宋杰 李世峰[2,3,4,5] 瞿素萍 LIAO Duosi;PENG Lyuchun;LIN Kaiwen;XIE Weijia;SONG Jie;LI Shifeng;QU Suping(Graduate School of Southwest Forestry University, Kunming 650224, China;Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China;National Engineering Research Center for Ornamental Horticulture, Kunming 650205, China;Yunnan Flower Breeding Key Laboratory,Kunming 650205, China;Yunnan Flower Research and Development Center, Kunming 650205, China)

机构地区：[1]西南林业大学研究生院,云南昆明650224 [2]云南省农业科学院花卉研究所,云南昆明650205 [3]国家观赏园艺工程技术研究中心,云南昆明650205 [4]云南省花卉育种重点实验室,云南昆明650205 [5]云南花卉技术工程研究中心,云南昆明650205

出　　处：《云南农业大学学报（自然科学版）》2019年第5期826-832,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金(31760230,31560225);云南省科技计划项目(2015BB013,2018BB010);云南省技术创新人才培养配套项目(2014-HB057);云岭产业领军人才项目(云发改人事[2014]1782号)

摘　　要：【目的】为了解两种黄花高山杜鹃(Bob’s yellow和黄杯杜鹃)组织培养的不同阶段对光质需求的规律,从而提高种苗质量及生产效率,促进其推广应用和保护。【方法】以两种黄花高山杜鹃组培苗为材料,在8种LED光质和传统荧光灯下进行增殖和生根培养,比较其增殖系数、增殖苗和生根苗叶绿素含量、生根率及生根苗株高等5个指标。【结果】纯红光最适宜Bob’s yellow增殖和生根,增殖系数为8.53,生根率为93.33%;纯蓝光适宜黄杯杜鹃增殖,增殖系数为4.2;红蓝光(2RB)处理下黄杯杜鹃生根效果最好,生根率为83.33%;红蓝绿光对两种高山杜鹃增殖均有抑制作用,但促进叶绿素合成。【结论】不同光质对两种黄花高山杜鹃组培苗增殖和生根的影响不同,同一基因型增殖和生根所需的光质配比也不相同。针对高山杜鹃不同基因型及不同的生长阶段,探索适宜的光质类型,可提高种苗质量及生产效率。[Purpose]To understand the demand for light quality of two genotypes of alpine Rhododendron in different stages when they are growing, so as to improve the quality and production efficiency of seedlings as well as facilitate its popularization, application and protection.[Method]The seedlings of two genotypes of alpine Rhododendron (Bob’s yellow and R. wardii W. W. Smith were growing under the conditions of 8 kinds of LED light and traditional fluorescent lamps. The effects of LED light quality on their proliferation and rooting was manifested by comparing the multiplication coefficient, the chlorophyll content of the proliferating and rooting seedlings, the rooting rate and the height.[Result]The pure red light was the most suitable for proliferation and rooting of Bob’s yellow. The multiplication coefficient was 8.53, and the rooting rate was 93.33%. The pure blue light was suitable for the proliferation of R. wardii and the multiplication coefficient was 4.2. 2RB was the best for R. wardii with rooting rate up to 83.33%. The red, blue and green light restricted the proliferation of these two Rhododendron, but helped chlorophyll synthesis.[Conclusion]The effects of different LED light quality on the proliferation and rooting of two genotypes of Rhododendron were different. For one otype, the optimal LED light quality was different during proliferation and rooting. To explore the suitable LED light quality, in view of different genotypes and different growth stages of Rhododendron, it could promote the seedling quality and production efficiency of Rhododendon.

关 键 词：高山杜鹃 组培苗 光质 增殖 生根 叶绿素 

分 类 号：S685.21[农业科学—观赏园艺]