1-磷酸鞘氨醇受体3调控RhoA/Rho激酶途径影响糖尿病ED大鼠勃起功能的初步研究  

Expession of sphingosine-1-phosphate receptor 3 in regulating RhoA/Rho pathway in erectile dysfunction rats with streptozotocin-induced type Ⅰ diabetes

作　　者：文博[1] 矫阳田 冯海航 李瑞[2] 周炳炎 王涛[2] 饶可[2] WEN Bo;JIAO Yangtian;FENG Haihang;LI Rui;ZHOU Bingyan;WANG Tao;RAO Ke(Department of Urology, People’s Hospital of Baoan District, Southern Medical University, Shenzhen 518101, China)

机构地区：[1]南方医科大学附属深圳宝安医院泌尿外科,518101 [2]华中科技大学同济医学院附属同济医院泌尿外科华中科技大学同济医学院附属同济医院泌尿外科研究所

出　　处：《现代泌尿生殖肿瘤杂志》2019年第4期233-237,共5页Journal of Contemporary Urologic and Reproductive Oncology

基　　金：深圳市科技计划项目（JCYJ20160427190559022）;国家自然科学基金（81200435）

摘　　要：目的探讨1-磷酸鞘氨醇受体3(S1PR3)在Ⅰ型糖尿病所致勃起功能障碍(ED)大鼠阴茎海绵体组织中的表达,为糖尿病ED的机制研究提供依据。方法18只正常8周龄雄性SD大鼠随机分为正常对照组和糖尿病组,建模8周后,测定2组大鼠阴茎海绵体内压/平均动脉压,采用免疫组化、PCR技术和Western blot方法检测S1PR3在2组大鼠阴茎海绵体组织中的表达水平,使用Western blot检测2组大鼠阴茎海绵体组织中RhoA、ROCK1和ROCK2的表达水平。结果与正常对照组相比,糖尿病组大鼠勃起功能明显降低(P<0.05);糖尿病大鼠阴茎海绵体组织中S1PR3 mRNA及其蛋白、RhoA、ROCK1和ROCK2蛋白的表达水平均明显升高(P<0.05)。结论Ⅰ型糖尿病可诱导大鼠ED,其原因可能与大鼠阴茎海绵体组织中S1PR3表达上调,进而激活RhoA/Rho激酶通路有关。Objective To investigate the mechanism of sphingosine-1-phosphate receptor 3 (S1PR3) in the cavernous tissues of diabetes mellitus-induced erection dysfunction (ED) rats. Methods Eighteen eight-week-old healthy male SD rats were randomly divided into 2 groups: Control group (CO, n =6);Diabetes mellitus group (DM, n =12). Diabetes rats were constructed, after 8 weeks, the maximum intracavernous pressure/mean arterial pressure were determined and the expression of S1PR3 were detected in the penis by IHC, PCR and Western blot,and the expression of RhoA, ROCK1 and ROCK2 in rat penile cavernous tissue were detected by Western blot. Results The erectile function significantly decreased in DM rats, compared to that of age-matched control rats ( P <0.05);the expression level of S1PR3, RhoA, ROCK1 and ROCK2 were higher in DM group than those in CO ( P <0.05). Conclusions Type Ⅰ DM can induce ED in rats, which may be related to the up-regulation of S1PR3 expression and the activation of RhoA/Rho kinase pathway in rat penile cavernous tissue.

关 键 词：1-磷酸鞘氨醇受体3 Rho相关激酶 勃起功能障碍 糖尿病 

分 类 号：R587.2[医药卫生—内分泌] R-332[医药卫生—内科学] R698.1[医药卫生—临床医学]