Effect of hydraulic retention time and pH on oxidation of ferrous iron in simulated ferruginous acid mine drainage treatment with inoculation of iron-oxidizing bacteria  

作　　者：Jun-hui Fan Xing-yu Liu Qi-yuan Gu Ming-jiang Zhang Xue-wu Hu 

机构地区：[1]GRIMAT Engineering Institute Co., Ltd., Beijing 101407, China [2]School of Metallurgical and Ecological Engineering, University of Science and Technology Beijing, Beijing 100083, China

出　　处：《Water Science and Engineering》2019年第3期213-220,共8页水科学与水工程（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant No.U1402234);the Guangxi Scientific Research and Technology Development Plan(Grants No.GuikeAB16380287 and GuikeAB17129025);the Public Welfare Fund of the Ministry of Environmental Protection of China(Grant No.201509049);the Program of International S & T Cooperation(Grant No.2016YFE0130700);the Fund of the General Research Institute for Nonferrous Metals(Grants No.53321 and 53348)

摘　　要：The effect of hydraulic retention time (HRT) and pH on the biooxidation of ferrous iron during simulated acid mine drainage (AMD) treatment was investigated.The simulated AMD was highly acidic (pH 2.5), rich in iron (about 1700 mg/L) and copper (about 200 mg/L), and contained high concentrations of sulfate (about 4700 mg/L).The biooxidation of ferrous iron was studied in a laboratory-scale upflow packed bed bioreactor (PBR).The HRT was shortened stepwise from 40 h to 20 h, 13 h, and 8 h under the acidic environment at a pH value of 2.2.Then, the influent pH value was changed from 2.2 to 1.2 at a constant suitable HRT.Physiochemical and microbial community structure analyses were performed on water samples and stuffing collected from the bioreactor under different conditions.The results indicate that the efficiency of ferrous iron oxidation gradually decreased with the decrease of HRT, and when the HRT exceeded 13 h, ferrous iron in AMD was almost completely oxidized.In addition, the best efficiency of ferrous iron oxidation was achieved at the influent pH value of 1.8.Microbial community structure analyses show that Leptospirillum is the predominant genus attached in the bioreactor, and low influent pH values are suitable for the growth of Leptospirillum.The effect of hydraulic retention time(HRT) and pH on the biooxidation of ferrous iron during simulated acid mine drainage(AMD)treatment was investigated.The simulated AMD was highly acidic(pH 2.5),rich in iron(about 1700 mg/L) and copper(about 200 mg/L),and contained high concentrations of sulfate(about 4700 mg/L).The biooxidation of ferrous iron was studied in a laboratory-scale upflow packed bed bioreactor(PBR).The HRT was shortened stepwise from 40 h to 20 h,13 h,and 8 h under the acidic environment at a pH value of 2.2.Then,the influent pH value was changed from 2.2 to 1.2 at a constant suitable HRT.Physiochemical and microbial community structure analyses were performed on water samples and stuffing collected from the bioreactor under different conditions.The results indicate that the efficiency of ferrous iron oxidation gradually decreased with the decrease of HRT,and when the HRT exceeded 13 h,ferrous iron in AMD was almost completely oxidized.In addition,the best efficiency of ferrous iron oxidation was achieved at the influent pH value of 1.8.Microbial community structure analyses show that Leptospirillum is the predominant genus attached in the bioreactor,and low influent pH values are suitable for the growth of Leptospirillum.

关 键 词：Acid mine drainage Iron-oxidizing bacteria BIOOXIDATION of FERROUS iron Hydraulic RETENTION time INFLUENT PH Microbial community analyses 

分 类 号：TV[水利工程]