共聚物P(NIPAM-co-AAEA)的合成及其抗菌凝胶涂层的性能  

Synthesis of Copolymer P(NIPAM-co-AAEA) and Properties of the Antibacterial Hydrogel Coating

作　　者：朱凯 朱保宁[1] 李莉莉[2] ZHU Kai;ZHU Baoning;LI Lili(Department of Environmental Science and Engineering, College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China;CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST), Beijing 100190, China)

机构地区：[1]北京化工大学化学工程学院环境科学与工程系,北京100029 [2]国家纳米科学中心,纳米生物效应与安全性重点实验室,北京100190

出　　处：《功能高分子学报》2019年第5期640-646,共7页Journal of Functional Polymers

基　　金：国家自然科学基金(51873045,31671028,21838001);中国科学院青年创新促进会(2017053)

摘　　要：首先,通过调控N-异丙基丙烯酰胺(NIPAM)与N-羟乙基丙烯酰胺(HEAA)单体的投料比,采用可逆加成断裂链转移聚合(RAFT)反应得到共聚物P(NIPAM-co-HEAA);然后,该共聚物与丙烯酰氯反应得到HEAA侧链双键功能化的共聚物P(NIPAM-co-AAEA);最后,P(NIPAM-co-AAEA)与双键功能化抗菌肽通过一步光交联反应在聚二甲基硅氧烷(PDMS)基底上形成温敏凝胶涂层。采用飞行时间质谱、核磁共振氢谱、红外光谱、扫描电镜、水接触角、荧光成像等手段对共聚物和凝胶涂层的结构和性能进行了表征。结果表明:当n(NIPAM)∶n(HEAA)=87∶13时,P(NIPAM-co-AAEA)的低临界溶解温度(LCST)为36.6℃,接近人体体温37℃,有望实现体温条件下的温敏响应;同时制备的温敏凝胶涂层具有较好的抗细菌黏附能力和良好的抗菌性能。Firstly, the copolymer poly[(N-isopropyl acrylamide)-co-(N-(2-hydroxyethyl)acrylamide)](P(NIPAM-coHEAA)) was obtained by reversible addition fragmentation chain transfer(RAFT) polymerization reaction at different feed ratio of NIPAM/HEAA. Then, the double bond functionalized copolymer poly[(N-isopropyl acrylamide)-co-(2-acrylamidoethyl acrylate)](P(NIPAM-co-AAEA)) was obtained by coupling with acryloyl chloride. Finally, thermo-sensitive hydrogel coating was fabricated by ‘one step ’ photocrosslinking reaction of thermo-sensitive copolymer P(NIPAM-coAAEA) and double bond functionalized antibacterial peptides(AMPs) on polydimethylsiloxane(PDMS). The thermosensitive copolymers were characterized by proton nuclear magnetic resonance(1 H-NMR) to identify the ratio of polymeric monomers. The AMPs were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF-MS). Then, the modification of the hydrogel on the substrate and the morphology were characterized by fourier transform infrared spectroscopy(FT-IR) and scanning electron microscope(SEM), respectively. The thermosensitivity of the hydrogel coatings were confirmed by static water contact angle(WCA) as a function of temperature variation. Finally, the antifouling and antibacterial properties of the hydrogel coating were validated by bacterial LIVE/DEAD viability assays characterized by Confocal Laser Scanning Microscope(CLSM) and colony counting assays. The results showed that when the feed ratio of NIPAM/HEAA was controlled at the optimized value of 87∶13, the LCST of P(NIPAMco-AAEA) was determined as 36.6 ℃, which was close to human body temperature 37 ℃. It is expected that these copolymers would present a thermo responsiveness under body temperature. Meanwhile, the prepared thermo-sensitive hydrogel coating exhibited expeted antifouling property and antibacterial activity.

关 键 词：温敏聚合物 抗菌肽 凝胶涂层 抗菌活性 抗黏附 

分 类 号：O63[理学—高分子化学]