罗汉松茎中总黄酮的提取工艺优化及其含量测定  

Optimization of extraction technology and content determination of total flavonoids from Podocarpus macrophyllus(Thunb.) D. Don

作　　者：韦锦梅 陆梅元 韦秀梅[3] 丘琴 黄凤音 陈科成 甄汉深 WEI Jin-mei;LU Mei-yuan;WEI Xiu-mei;QIU Qin;HUANG Feng-yin;CHEN Ke-cheng;ZHEN Han-shen(School of Pharmaceutical Sciences,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Food and Drug Evaluation and Inspection Center,Nanning 530200,China;Liuzhou Traditional Chinese Medicine Hospital,Liuzhou 545001,Guangxi,China)

机构地区：[1]广西中医药大学药学院,南宁530020 [2]广西壮族自治区食品药品审评查验中心,南宁530020 [3]柳州市中医院,广西柳州545001

出　　处：《湖北农业科学》2019年第18期98-101,共4页Hubei Agricultural Sciences

基　　金：壮瑶药协同创新中心项目[桂教科研(2013)20号];广西壮瑶药重点实验室项目[桂科基字(2014)32号];广西重点学科壮药学项目[桂教科研(2013)16号];广西八桂学者中药创新理论与药效研究(J13162);广西高校中药提取纯化与质量分析重点实验室项目[桂教科研(2014)6号];国家中医药管理局科研三级实验室中(壮)药化学与质量分析实验室项目[国中医药发2009(21)号]

摘　　要：采用超声提取法提取罗汉松[Podocarpus macrophyllus(Thunb.)D.Don]茎中的总黄酮,通过单因素试验方法考察了提取方法、提取溶剂体积分数、提取溶剂体积、提取时间对总黄酮含量的影响,采用正交试验优化提取工艺条件,筛选出罗汉松茎中总黄酮的最佳提取工艺。结果表明,最佳提取工艺为70%乙醇、超声提取1.0h、料液比1∶20(m/V)。超声提取法提取罗汉松茎中总黄酮含量,该方法重复性较好、稳定、可行。The total flavonoids of the stem of Podocarpus macrophyllus(Thunb.)D.Don was extracted by ultrasonic extraction,and the effects of extraction methods,solvent concentration,extraction sdvent volume and extraction time on total flavonoids were investigated by single factor test.Orthogonal experiment was used to optimize the extraction process and select the best extraction process of total flavonoids from the stem of Podocarpus macrophyllus(Thunb.)D.Don.The results showed that the best extraction technology was 70%ethanol,ultrasonic extraction of 1.0 h,and the ratio of material to liquid 1∶20(m/V).The ultrasonic extraction method can be used to extract the total flavonoids of the stem of Podocarpus macrophyllus(Thunb.)D.Don.The method has good reproducibility,stability and feasibility.

关 键 词：罗汉松[Podocarpus macrophyllus(Thunb.)D.Don] 茎 总黄酮 提取工艺 

分 类 号：R282.2[医药卫生—中药学]