老年原发性干燥综合征患者血清miR-146a和miR-155表达及临床意义  

Expressions and clinical significances of serum miR-146a and miR-155 in elderly patients with primary Sjogren’s syndrome

作　　者：朱丽娟 张振山 夏楠楠 张伟峰 郑晶[2] 孟飞龙 杨磊 沈龙 ZHU Li-Juan;ZHANG Zhen-Shan;XIA Nan-Nan(Department of Rheumatology and Immunology, Zhengzhou Central Hospital Affiliated to Zhengzhou University , Zhengzhou 450007, Henan, China)

机构地区：[1]郑州大学附属郑州中心医院风湿免疫科,河南郑州450007 [2]中山大学附属第五医院风湿免疫科 [3]厦门大学

出　　处：《中国老年学杂志》2019年第20期5020-5024,共5页Chinese Journal of Gerontology

基　　金：国家自然科学基金项目(No.81571585)

摘　　要：目的 探究miR-146a和miR-155在老年原发性干燥综合征(PSS)患者血清中的表达及临床意义。方法 老年PSS患者80例(疾病组)和同期健康体检者80例(对照组)为研究对象,采用实时荧光定量PCR法检测血清miR-146a、miR-155表达水平,采用阻抗法检测血清白细胞(WBC)含量,采用免疫比浊法测定血清免疫球蛋白(Ig)G抗体水平,采用间接免疫荧光法检测血清抗核抗体(ANA),采用免疫组化法检测血清抗SSA、抗SSB抗体,采用Pearson法分析miR-146a、miR-155及二者分别与WBC、IgG的相关性;采用受试者工作特征(ROC)曲线分析检测miR-146a、miR-155表达水平对PSS诊断价值。结果 与对照组比较,疾病组血清中miR-146a表达水平明显升高(P<0.001),miR-155表达水平明显降低(P<0.001),血清中WBC数量显著减少(P<0.001),IgG水平显著增加(P<0.001);与抗ANA阴性组、抗SSA阴性组、抗SSB阴性组比较,抗ANA阳性、抗SSA阳性组、抗SSB阳性组miR-146a表达水平显著升高,miR-155表达水平显著降低(P<0.001);PSS患者血清中miR-146a与miR-155表达呈显著负相关(r=-0.718,P<0.05),与WBC呈显著负相关(r=-0.694,P<0.05),与IgG呈显著正相关(r=0.690,P<0.05);PSS患者血清中miR-155与WBC呈显著正相关(r=0.566,P<0.05),与IgG呈显著负相关(r=-0.693,P<0.05);血清miR-146a、miR-155表达水平诊断PSS疾病的曲线下面积(AUC)为0.831、0.834,截断值为0.625、0.651,特异度为0.825、0.838,灵敏度为0.800、0.813;二者联合诊断PSS疾病的AUC为0.884,特异度为0.925,灵敏度为0.763。结论 miR-146a在PSS患者血清中高表达,miR-155低表达,二者呈负相关,可能参与PSS发生发展过程,对PSS早期诊断有一定参考价值。Objective To investigate the expressions and clinical significances of microRNA-146a (miR-146a) and microRNA-155 (miR-155) in the serum of elderly patients with primary Sjogren's syndrome(PSS). Methods 80 elderly patients with PSS and 80 healthy people (control group) were collected from January 2016 to January 2019. Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the expression levels of serum miR-146a and miR-155, the content of WBC in serum was measured by impedance method, serum IgG antibody level was determined by immunoturbidimetry, anti-ANA antibody in serum was detected by indirect immunofluorescence assay, serum anti-SSA and anti-SSB antibodies were detected by immunohistochemistry. Pearson method was used to analyze the correlation between miR-146a and miR-155 and their correlations with WBC, IgG, respectively. ROC curve analysis was used to detect the expression levels of miR-146a and miR-155 in the diagnosis of PSS. Results Compared with that of control group, the level of serum miR-146a of PSS group was significantly increased ( P <0.05), while the level of miR-155 was significantly decreased ( P <0.05). The number of WBC in serum of patients with PSS was decreased significantly, while the level of IgG was increased significantly ( P <0.05). Compared with that of anti-ANA negative group, the expression level of miR-146a in the anti-ANA positive group was increased significantly, and the expression level of miR-155 was decreased significantly (P < 0.05). Compared with that of anti-SSA negative group, the expression level of miR-146a in anti-SSA positive group was increased significantly, and the expression level of miR-155 was decreased significantly ( P <0.05). Compared with that of anti-SSB negative group, the expression level of miR-146a in anti-SSB positive group was increased significantly, and the expression level of miR-155 was decreased significantly ( P <0.05). Pearson analysis showed that there was a negative correlation between the expression of miR-146a and the express

关 键 词：miRNA-146a miRNA-155 原发性干燥综合征 

分 类 号：R593[医药卫生—内科学]