蛴螬提取物对兔光损伤视网膜变性Fas、FasL表达的影响  

Effects of Lycium Barbarum L.Extract on Expression of Fas and Fasl in Rabbits with Photodamage Retinal Degeneration

作　　者：蒋鹏飞[1,4] 吴大力 彭俊[3,4] 彭清华 Jiang Pengfei;Wu Dali;Peng Jun;Peng Qinghua(Hunan University of Traditional Chinese Medicine,Changsha 410208,China;The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning 530023,China;First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410007,China;KeyLaboratory of Traditional Chinese Medicine for Prevention and Treatment of Eye,Ear,Nose and Throat Diseases in Hunan Province,Changsha 410208,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]广西中医药大学第一附属医院,广西南宁530023 [3]湖南中医药大学第一附属医院,湖南长沙410007 [4]中医药防治眼耳鼻咽喉疾病湖南省重点实验室,湖南长沙410208

出　　处：《亚太传统医药》2019年第9期15-18,共4页Asia-Pacific Traditional Medicine

基　　金：国家自然科学基金资助项目(81804150);湖南省中医药科研基金资助项目(2010012);湖南省教育厅科研基金资助项目(09C729);湖南中医药大学科研基金资助项目(3601010157);湖南中医药大学中医学国内一流建设学科资助项目;中医药防治五官科疾病湖南省重点实验室建设项目(2017TP1018);中央财政支持地方高校建设项目;国家中医药管理局中医眼科学重点学科建设项目;湖南省中医五官科学重点学科建设项目;湖南省研究生科研创新项目(CX2018B497)

摘　　要：目的:探讨蛴螬提取物对兔光损伤视网膜变性Fas、Fasl表达的影响.方法:将30只实验性兔随机分成5组,分别为空白组、模型组、蛴螬低剂量组、蛴螬中剂量组、蛴螬高剂量组,每组6只动物,12只眼.除空白组外,其余4组均建立光损伤视网膜变性模型,在给药14d处死全部兔子后,取材做Fas、FasL免疫组化检测,采用计算机系统图像分析结果并进行统计分析,Western Blot法检测各组视网膜组织中Fas、FasL蛋白表达.结果:与模型对照组相比,蛴螬中、高剂量治疗组的Fas、FasL积分光密度与蛋白表达均降低,差异有统计学意义(P<0.05).结论:蛴螬通过降低凋亡因子Fas、FasL的表达,抑制细胞凋亡受体信号通路来发挥保护视网膜的作用,中、高剂量组效果较好.Objective:To investigate the effects of alfalfa extract on the expression of Fas and Fasl in rabbits with photodamaged retinal degeneration.Methods:Thirty experimental rabbits were randomLy divided into 5 groups:blank group,model group,low dose group,middle dose group,and high dose group,each group consisting of 6 animals and 12 eyes.Except for the blank group,the other four groups established a model of light loss retinal degeneration.After all the rabbits were sacrificed for 14 days,the Fas and FasL immunohistochemistry were performed,and the computer system image analysis resuLts were performed and statistical analysis was performed.Western Blot assay for Fas and FasL protein expression in retinal tissues of each group.Results:Compared with the model control group,the Fas and FasL integral optical density and protein expression of the middle and high dose treatment groups were decreased,and the difference was statistically significant (P<0.05).Conclusion:Grubprotects the retina by decreasing the expression of Fas and FasL and inhibiting the apoptotic death receptor signaling pathway and the medium and high dose groups performed better.

关 键 词：蛴螬提取物 光损伤 FAS FASL 

分 类 号：R285.5[医药卫生—中药学]