Genetic effects and plant architecture influences on outcrossing rate in soybean  

作　　者：YAN Hao ZHANG Jing-yong ZHANG Chun-bao PENG Bao ZHANG Wei-long WANG Peng-nian DING Xiao-yang LIU Bao-hui FENG Xian-zhong ZHAO Li-mei 

机构地区：[1]Key Laboratory of Soybean Molecular Design Breeding,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Changchun 130102,P.R.China [2]Soybean Research Institute,Jilin Academy of Agricultural Sciences/National Engineering Research Center for Soybean,Changchun 130033,P.R.China [3]University of Chinese Academy of Sciences,Beijing 100049,P.R.China

出　　处：《Journal of Integrative Agriculture》2019年第9期1971-1979,共9页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation for Young Scientists of China (31301399);the Major Project for Science and Technology Development of Jilin Province, China (20170201001NY);the Agricultural Science Technology Innovation Project of Jilin Province, China (CXGC2017TD002)

摘　　要：Outcrossing rate is an important determinant of cytoplasmic male sterile(CMS)breeding and hybrid seed production for heterosis in soybean.Parental lines with a high outcrossing rate were screened for backcross breeding to obtain the high outcrossing rate maintenance B-lines and sterile A-lines.Application in production practices will help to increase hybrid soybean production.In this study,JLCMS82B and JLCMS89B were selected as parents for the construction of outcrossing rate segregation populations,and the progeny-array approach(PAA)and glyphosate resistant gene markers were used to determine outcrossing rates.We found that:(1)The outcrossing rate between JLCMS82B and JLCMS89B was significantly different;(2)the outcrossing rate of the F2 segregating populations was a quantitative trait,though whether an additive or epistatic effect exists required analysis with a triple test intersection analysis;(3)agronomic traits correlated with outcrossing rate;outcrossing rate was the highest with plant height of about 84 cm,lower number of plant branches,earlier flowering time,larger angle between the branches and the main stem,and with more divergent plant morphology.Correlation analysis between agronomic traits and outcrossing rate can effectively guide the screening of parents with a high outcrossing rate.Outcrossing rate is an important determinant of cytoplasmic male sterile（CMS） breeding and hybrid seed production for heterosis in soybean. Parental lines with a high outcrossing rate were screened for backcross breeding to obtain the high outcrossing rate maintenance B-lines and sterile A-lines. Application in production practices will help to increase hybrid soybean production. In this study, JLCMS82B and JLCMS89B were selected as parents for the construction of outcrossing rate segregation populations, and the progeny-array approach（PAA） and glyphosate resistant gene markers were used to determine outcrossing rates. We found that:（1） The outcrossing rate between JLCMS82B and JLCMS89B was significantly different;（2） the outcrossing rate of the F2 segregating populations was a quantitative trait, though whether an additive or epistatic effect exists required analysis with a triple test intersection analysis;（3） agronomic traits correlated with outcrossing rate; outcrossing rate was the highest with plant height of about 84 cm, lower number of plant branches, earlier flowering time, larger angle between the branches and the main stem, and with more divergent plant morphology. Correlation analysis between agronomic traits and outcrossing rate can effectively guide the screening of parents with a high outcrossing rate.

关 键 词：SOYBEAN OUTCROSSING rate stem HEIGHT branch number initial BLOOM DATE 

分 类 号：S[农业科学]