滤泡调节性T细胞促进修复糖尿病损伤颈动脉内皮的作用及机制研究  

Role and mechanism of follicular regulatory T cells in promoting vascular endothelial repair in diabetic mouse

作　　者：熊雯 初红霞[2] 张宪党[1] 丁文宇[1] 张海清[3] XIONG Wen;CHU Hongxia;ZHANG Xiandang(Department of Endocrinology,Shandong Institute of Endocrine and Metabolic Disease,Ji' nan 250062,China)

机构地区：[1]山东省内分泌与代谢病研究所内分泌科,济南250062 [2]山东省烟台毓璜顶医院心血管内科 [3]山东省立医院内分泌科

出　　处：《中国糖尿病杂志》2019年第9期701-706,共6页Chinese Journal of Diabetes

摘　　要：目的 探讨滤泡调节性T细胞(Tfr细胞)促进修复糖尿病损伤颈动脉内皮的作用及机制.方法 构建糖尿病小鼠模型,构建小鼠颈动脉铁丝损伤模型.分为对照组(Con)、糖尿病组(DM)和Tfr细胞组(Tfr).流式细胞学检测Tfr细胞比例,ELISA法检测上清中血管内皮生长因子(VEGF)、白介素10(IL-10)和转化生长因子β(TGF-β)的浓度.HE染色和CD31免疫组织化学检测内皮化情况,流式细胞学检测外周血中内皮祖细胞(EPC)比例.结果 Con组外周血Tfr细胞的比例高于DM组(t=3.122,P=0.0108).Con组上清中VEGF、IL-10和TGF-β的浓度高于DM组(P<0.05或P<0.01).Tfr组移植48 h后,CD31阳性内皮化比例约为60%.Con组外周血EPC比例与DM及Tfr组比较(1.070%vs 0.288%vs 1.993%),差异有统计学意义(P<0.01).结论 糖尿病小鼠体内Tfr细胞数量和功能降低,Tfr细胞可通过诱导EPC动员,以促进糖尿病损伤颈动脉内皮的修复.Objective To investigate the effect and mechanism of follicular regulatory T cells (Tfr) on vascular endothelial repair. Methods Diabetic mouse model was established and the rats were divided into control (Con), diabetes (DM) and Tfr groups. The proportion of Tfr cells was detected by flow cytometry. ELISA method was used to detect the concentration of vascular endothelial growth factor (VEGF), IL-10 and TGF-β in the supernatant. The mouse carotid artery wire injury model was constructed. The endothelializa- tion was detected by HE staining and CD31 immunohistochemistry. The flow cytometry was used to detect the proportion of endothelial progenitor cells (EPC) in peripheral blood. Results The proportion of Tfr cells in peripheral blood was 0. 1209% in control group and 0.02917% in diabetic group (t=3.122, P= 0. 0108). The concentrations of VEGF, IL-10 and TGF-P in the supernatant were higher in control group than thse in diabetes group (P<0. 05 or P<0. 01). After 48 hours of transplantation, the proportion of CD31 positive endothelialization reached about 60% in Tfr cell group. The proportion of EPC in the periph eral blood was 1.070% in control group, 0.288% in diabetic group and 1.993% in Tfr cell group (t= 8.442, P<0. 01). Conclusion The number and function of Tfr cells in diabetic mice are significantly reduced. Tfr cells can promote the endothelial repair of the carotid artery by inducing EPC mobilization.

关 键 词：滤泡调节性T细胞 糖尿病 内皮化 内皮祖细胞 

分 类 号：R587.2[医药卫生—内分泌]