高位脊髓损伤大鼠心肌GLUT4膜转位的变化  

Changes in myocardial GLUT4 membrane translocation in rats with high-level spinal cord injury

作　　者：黄世宏 陈辉[2] 陈丽菲[2] 张燕萍 李立芊 黄钦凤 Huang Shihong;Chen Hui;Chen Lifei;Zhang Yanping;Li Liqian;Huang Qinfeng(Department of Anesthesiology,Affiliated Fuzhou First Hospital of Fujian Medical University,Fuzhou 350009,China;Department of Anesthesiology,First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China)

机构地区：[1]福建医科大学附属福州市第一医院麻醉科,350009 [2]福建医科大学附属第一医院麻醉科,福州350005

出　　处：《中华麻醉学杂志》2019年第6期750-753,共4页Chinese Journal of Anesthesiology

基　　金：福建省卫生厅青年科研基金(2018-2-19).

摘　　要：目的评价高位脊髓损伤大鼠心肌葡萄糖转运蛋白4(GLUT4)膜转位的变化.方法清洁级健康成年雄性SD大鼠36只,体重250~300 g.采用随机数字表法分为3组:对照组(C组,n=6)、假手术组(S组,n=6)和高位脊髓损伤组(SCI组,n=24).采用改良的Allens打击法建立大鼠高位脊髓损伤模型,S组暴露脊髓后不进行打击.C组和S组每组取6只大鼠、SCI组分别于打击后4、12、24及48 h(T1-4)时随机取6只大鼠,采集腹主动脉血样,测定血清CK和CK-MB活性,然后处死大鼠,取心肌组织,透射电镜下观察超微结构,测定心肌ATP重量比,采用Western blot法测定胰岛素受体底物1的酪氨酸磷酸化水平(p-IRS1-Tyr)和细胞膜GLUT4的表达水平.结果与C组和S组比较,SCI组T1-4时血清CK和CK-MB活性升高,心肌ATP重量比降低,心肌细胞膜GLUT4表达下调,T2,3时心肌p-IRS1-Tyr表达下调(P<0.05),发生心肌病理学损伤;C组和S组上述各指标比较差异无统计学意义(P>0.05).结论高位脊髓损伤大鼠心肌能量代谢障碍的机制可能与GLUT4膜转位减少有关.Objective To evaluate the changes in myocardial glucose transporter 4 ( GLUT4 ) membrane translocation in the rats with high-level spinal cord injury ( SCI ). Methods Thirty-six clean-grade healthy adult male Sprague-Dawley rats, weighing 250-300 g, were divided into 3 groups using a random number table method: control group (group C, n=6), sham operation group (group S, n=6) and high-level SCI group (group SCI, n=24). The model of SCI was established by a modified Allen's method in anesthetized rats. The spinal cord was only exposed in group S. Six rats were selected in C and S groups and at 4, 12, 24 and 48 h after SCI ( T1-4 ) in group SCI, and blood samples were taken from the abdominal aorta to measure the activities of serum creatine kinase and creatine kinase isoenzyme-MB. The rats were then sacrificed, and myocardial specimens were collected for microscopic examination of the ultra-structure ( with a transmission electron microscope) and for determination of ATP weight ratio, phosphoryla-tion of insulin receptor substrate-1 tyrosine and expression of GLUT4 in cell membrane ( by Western blot). Results Compared with C and S groups, the serum creatine kinase and creatine kinase isoenzyme-MB ac-tivities were significantly increased at T1-4 , the ATP weight ratio was decreased, the expression of GLUT4 in myocardial cell membrane was down-regulated, the expression of phosphorylated insulin receptor sub-strate-1 tyrosine in myocaradium was down-regulated at T2,3 (P<0. 05), and the pathological changes of myocardial tissues were found in group SCI. There was no significant difference in the indexes mentioned a-bove between group C and group S ( P>0. 05). Conclusion The mechanism of myocardial energy metabo-lism disorder may be related to the reduced membrane translocation of GLUT4 in the rats with high-level SCI.

关 键 词：脊髓损伤 心肌 葡萄糖转运蛋白4 

分 类 号：R651.2[医药卫生—外科学]