Alda-1通过激活线粒体乙醛脱氢酶2改善大鼠肺缺血再灌注损伤  

Attenuation of lung ischemia-reperfusion injury by Alda-1 via activating mitochondrial acetaldehyde dehydrogenase in rats

作　　者：张裕坚[1] 林婷婷[1] 夏芳芳[1] 董娇娇[1] 金周晟[1] 刘乐[1] ZHANG Yujian;LIN Tingting;XIA Fangfang;DONG Jiaojiao;JIN Zhousheng;LIU Le(Department of Anesthesiology,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou325015,China)

机构地区：[1]温州医科大学附属第一医院麻醉科

出　　处：《温州医科大学学报》2019年第10期744-747,共4页Journal of Wenzhou Medical University

基　　金：温州市科技计划项目(Y20160371)

摘　　要：目的:通过Alda-1激活线粒体乙醛脱氢酶2(ALDH2)活性,探讨激活该酶对大鼠肺缺血再灌注损伤的保护作用。方法:选择24只SD雄性大鼠,随机分为假手术组(Sham组)、缺血再灌注组(I/R组)和Alda-1+缺血再灌注组(Alda-1组)。实验结束取肺组织和动脉血标本,测各组肺组织的湿干重比(W/D)、HE染色观察肺泡结构;检测血浆及肺组织的丙二醛(MDA)含量、4-羟基壬烯醛(4-HNE)浓度;Western blot检测ALDH2的相对表达量及ELISA检测ALDH2的活性。结果:与Sham组相比,I/R组肺组织W/D值明显升高(P<0.05),肺泡结构明显破坏,血浆及肺组织的MDA、4-HNE明显增多(P<0.05),ALDH2的活性明显降低(P<0.05);与I/R组相比,Alda-1组肺组织W/D值显著下降(P<0.05),肺泡结构显著改善,血浆及肺组织的MDA、4-HNE显著减少(P<0.05),ALDH2的活性明显升高(P<0.05)。结论:Alda-1可通过激活ALDH2的活性来加速醛类物质代谢从而减轻肺缺血再灌注损伤。Objective: To investigate the protective effect of Alda-1 activated mitochondrial acetaldehyde dehydrogenase 2(ALDH2) on lung ischemia-reperfusion injury(LIRI) in rats. Methods: Twenty-four SD male rats were randomly divided as sham operation group(Sham group), ischemia-reperfusion group(I/R group) and Alda-1+ischemia-reperfusion group(Alda-1 group). The lung tissue and arterial blood samples were taken at the end of the experiment. The wet-to-dry weight ratio(W/D) of the lung tissue was measured;the alveolar structure was observed by HE staining;the malondialdehyde(MDA) and 4-hydroxynonenal(4-HNE) in plasma and lung tissue were detected;the content of ALDH2 was determined by Western blot and the activity of ALDH2 by ELISA. Results: Compared with the Sham group, the I/R group showed that the W/D value was significantly increased(P<0.05), the alveolar structure markedly destroyed, MDA and 4-HNE in the plasma and lung tissues remarkably improved(P<0.05), the activity of ALDH2 signally decreased(P<0.05). However, in the Alda-1 group, the W/D value was significantly decreased(P<0.05), the alveolar structure markedly improved, the MDA and 4-HNE of plasma and lung tissues remarkably decreased(P<0.05), and the activity of ALDH2 signally improved(P<0.05), compared with the I/R group. Conclusion: Alda-1 can accelerate the metabolism of aldehydes by activating ALDH2 to improve lung ischemia-reperfusion injury.

关 键 词：肺 再灌注损伤 Alda-1 乙醛脱氢酶2 丙二醛 4-羟基壬烯醛 

分 类 号：Q319.1[生物学—遗传学] R339.34[医药卫生—人体生理学]