亚硝化细菌在厌氧氨氧化过程中固碳路径研究  

Carbon Sequestration Pathway of Nitrosated Bacteria in Anaerobic Ammonium Oxidation Process

作　　者：刘小宁 王华琴[1] 王敦球[1] 张文杰[1] LIU Xiaoning;WANG Huaqin;WANG Dunqiu;ZHANG Wenjie(Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology,Guilin University of Technology,Guangxi CollaborativeInnovation Center for Water Pollution Control and Water Safety in Karst Area,Guilin University of Technology,Guilin 541004,China)

机构地区：[1]桂林理工大学广西环境污染控制理论与技术重点实验室广西岩溶地区水污染控制与用水安全保障协同创新中心

出　　处：《水处理技术》2019年第10期30-35,共6页Technology of Water Treatment

基　　金：国家自然科学基金资助项目(51668013,51638006);“八桂学者”建设工程专项经费;广西科技计划项目资助(桂科AD18126018);桂林市科学研究与技术开发计划项目(2016012303);广西矿冶与环境科学实验中心(KH2012ZD004);广西高等学校高水平创新团队及卓越学者计划项目(002401013001)

摘　　要：为研究厌氧氨氧化过程中亚硝化细菌(AOB)的固碳能力,在不同进水基质的条件下对亚硝化细菌进行模拟研究并确立优化运行条件,用13C同位素示踪法和实时荧光定量PCR确定亚硝化细菌的固碳路径。结果表明,NH4+^-N、HCO3^-、Ca^2+、Mg^2+的质量浓度分别为15、250、113、100 mg/L,微生物促进剂的体积分数1 ml/L时系统脱氮效果较好,亚硝酸盐积累率高达96.95%。13C同位素示踪发现污泥中的13C丰度明显增加,进水中投加的无机碳参与了微生物体内的碳代谢。实验确定了短程硝化细菌中存在遵循卡尔文循环固碳途径的功能基因,且该基因丰度对氮素的响应度比对其他基质大。In order to study the carbon sequestration ability of nitrosated bacteria(AOB)in anaerobic ammonium oxidation process,the nitrosation bacteria were simulated under different influent substrates and the optimal operation conditions was established.The carbon sequestration path of nitrosated bacteria were determined by 13C isotope tracing and real-time fluorescent quantitative PCR.The results showed that,when the mass concentration of NH4+^-N,HCO3^-,Ca^2+and Mg^2+,volume fraction of microbial promoters were 15 mg/L,250 mg/L,113 mg/L,100 mg/L and 1 ml/L,respectively,the system had good denitrification effect and the nitrite accumulation rate was as high as 96.95%.The 13 Cisotope tracer showed that the 13C abundance in the sludge increased significantly,and the inorganic carbon added in the influent participated in the carbon metabolism of the microorganism.The test identified a functional gene in the short-range nitrifying bacteria that follows the Calvin cycle carbon sequestration pathway,and the gene abundance is more responsive to nitrogen than to other matrices.

关 键 词：厌氧氨氧化 亚硝化细菌 基质 固碳 13C同位素丰度 基因丰度 

分 类 号：X703.1[环境科学与工程—环境工程]