硅钼蓝法测定硅烷化玻璃酸复合物中的有机硅含量  

Determination of Organosilicon in Silanol Hyaluronate Compound by Silicon Molybdenum Blue Spectrophotometry

作　　者：张治云 姜鹰雁 刘荣磊 刘亚方 陈建英 ZHANG Zhi-yun;JIANG Ying-yan;LIU Rong-lei;LIU Ya-fang;CHEN Jian-ying(Shandong Academy of Pharmaceutical Sciences, Jinan 250101, China;Shandong Provincial Key Laboratory of Mucosal and Transdermal Drug Delivery Technologies, Shandong Freda Pharmaceutical Group Co., Ltd., Jinan250101, China)

机构地区：[1]山东省药学科学院,山东济南250101 [2]山东福瑞达医药集团有限公司山东省黏膜与皮肤给药技术重点实验室,山东济南250101

出　　处：《食品与药品》2019年第5期363-366,共4页Food and Drug

基　　金：2018年度山东省重点研发计划（公益类专项，编号：2018GSF118045）

摘　　要：目的建立硅钼蓝分光光度法测定硅烷化玻璃酸复合物中有机硅含量的方法。方法硅烷化玻璃酸复合物经高温灰化和氢氧化钠熔融处理与钼酸铵反应生成硅钼酸,经抗坏血酸还原成硅钼蓝,810nm处测定吸光度,通过标准曲线法计算硅的含量。结果硅浓度在0.0988~0.484μg/ml范围内与吸光度呈良好线性关系;对照品溶液还原成硅钼蓝后吸光度在10~60min内稳定;平均加样回收率为98.52%(RSD=1.74%)。结论本方法准确、可靠、操作简便,可用于硅烷化玻璃酸复合物中有机硅的含量测定。Objective To establish a method for the determination of organosilicon content in silanol hyaluronate compound by silicon molybdenum blue spectrophotometry. Methods The silanol hyaluronate compound was subjected to high temperature ashing and melting treatment of sodium hydroxide and then reacted with ammonium molybdate to form silicomolybdic acid, which was reduced to silicon molybdenum blue by ascorbic acid. The absorbance was measured at 810 nm and the silicon content was calculated by standard curve method. Results The concentration of silicon in the range of 0.0988-0.484 μg/ml showed a good linear relationship with the absorbance. The absorbance of the reference solution was stable between 10-60 min after reduction to silicon molybdenum blue. The average recovery was 98.52 %(RSD=1.74 %). Conclusion This method is accurate, reliable and simple. It can be used to determine the content of organosilicon in silanol hyaluronate compound.

关 键 词：硅钼蓝分光光度法 硅烷化玻璃酸复合物 有机硅 

分 类 号：R917[医药卫生—药物分析学]