蔓荆子中木脂素类成分的分离与结构鉴定  

Isolation and structural identification of lignans from fruits of Vitex trifolia L.var. simplicifolia Cham.

作　　者：黄艳丽 张晗[1] 郭颖 李安平 姚进龙 邓雁如[1] 房士明[1] HUANG Yanli;ZHANG Han;GUO Ying;LI Anping;YAO Jinlong;DENG Yanru;FANG Shiming(Tianjin University of Traditional Chinese Medicine,Key Laboratory of Traditional Chinese Medicinal Chemistry and Analytical Chemistry of Tianjin,Tianjin 300193,China;Tianjin Nankai Hospital,Tianjin 300100,China)

机构地区：[1]天津中医药大学,天津市中药化学与分析重点实验室,天津301617 [2]天津南开医院,天津300100,瀚盟测试科技(天津)有限公司,天津300457

出　　处：《天津中医药大学学报》2019年第5期496-500,共5页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：国家高技术研究发展计划("863"计划)资助项目(2013AA093001)

摘　　要：[目的]对蔓荆子中的木脂素类成分进行研究。[方法]采用正相硅胶、Sephadex LH-20、MCI GEL (MCI)、反相ODS(ODS)等柱色谱及高效液相色谱手段进行分离纯化,并通过理化性质与波谱分析方法鉴定化合物的结构。[结果]从蔓荆子95%乙醇提取物中分离得到7个木脂素类单体成分,分别为dihydrodehydrodiconiferyl alcohol (1),ficusal (2),(7R,8S)-dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucopyranoside (3),dihydrodehydrodiconiferyl alcohol-β-D-(2′-O-p-hydroxybenzoyl)glucoside (4),(7R,8R)-7,8-dihydro-9′-hydroxyl-3′-methoxyl-8-hydroxymethyl-7-(4-hydroxy-3-methoxyphenyl)-1′-benzofuranpropanol 9′-O-β-D-glucopyranoside (5),松脂醇(pinoresinol)(6),1-syringaresinol (7)。[结论]化合物 5 为首次从牡荆属中分离得到,化合物 6 和7 为首次从该植物中分离得到的化合物。[Objective] To study the lignans from the fruits of Vitex trifolia L. var. simplicifolia Cham.[Methods] The chemical compounds were isolated and purified by Silica gel column,Sephadex LH-20,MCI,ODS and PHPLC (Preparative high performance liquid chromatography,PHPLC) chromatorgraphies and identified by chemical and spectral analysis 1H,13C-NMR (Nuclear magnetic resonance,NMR),COSY (Correlation spectroscopy,COSY),HMQC (1H detected heteronuclear multiple quantum coherence,HSQC),HMBC (1H detected heteronuclear multiple bond correlation,HMBC) and MS (Mass spectrometer,MS).[Results] Seven lignan monomers were isolated from 95% ethanol extract of Vitex trifolia L. var. simplicifolia Cham. and identified as dihydrodehydrodiconiferyl alcohol (1),ficusal (2),(7R,8S)-dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucopyranoside (3),dihydrodehydrodiconiferyl alcohol-β-D-(2’-O-p-hydroxybenzoyl) glucoside (4),(7R,8R)-7,8-dihydro-9’-hydroxyl-3'-methoxyl-8-hydroxymethyl-7-(4-hydroxy-3-methoxyphenyl)-1’-benzofuranpropanol 9’-O-β-D-glucopyranoside (5),pinoresinol (6),1-syring- aresinol (7).[Conclusion] Compound 5 was obtained from Vitex genus firstly,and compounds 6,7 were isolated from the plant for the first time.

关 键 词：蔓荆子 木脂素 化学成分 结构鉴定 

分 类 号：R284.1[医药卫生—中药学]