苏太猪源TTP基因的克隆及其遗传进化分析  

Cloning and Genetic Evolution Analysis of TTP Gene from Sutai Procine

作　　者：陈荣 张喜懿 田浪 温贵兰[1,2] 祝景 晏旭红 付尚林 Chen Rong;Zhang Xiyi;Tian Lang;Wen Guilan;Zhu Jing;Yan Xuhong;Fu Shanglin(Institute of Animal Diseases, Guizhou University, Guiyang Guizhou 550025, China;Animal Biological Engineering Technology Research Center in Guizhou Province, Guiyang Guizhou 550016, China)

机构地区：[1]贵州大学动物疫病研究所,贵州贵阳550025 [2]贵州省动物生物制品工程技术研究中心,贵州贵阳550016

出　　处：《贵州畜牧兽医》2019年第5期10-14,共5页Guizhou Journal of Animal Husbandry & Veterinary Medicine

基　　金：“P-body在PRRSV复制和持续感染过程中的作用机制研”国家自然科学基金项目(No.31460668);“NSP2亚类在PRRSV复制过程中的作用机制研究”贵州省科学技术基金项目(黔科合J字[2015]2046);贵州大学“SRT”计划项目[贵大SRT(2018)183号]

摘　　要：为了分析苏太猪源Tristetraproin(TTP)基因CDS序列和遗传进化关系,试验根据GenBank公布的野猪源TTP基因设计1对特异性引物,通过RT-PCR从苏太猪血液中扩增出TTP基因CDS区,并进行克隆和遗传进化分析。结果:扩增所得的TTP基因CDS长度为981bp,共编码326个氨基酸。同源性分析表明:该基因核苷酸编码序列同源性与GenBank中公布的野猪源TTP基因一致性最高,为99.8%。系统进化树显示:该基因与野猪源TTP基因在遗传进化树上位于同一分支,亲缘关系最近;与白鱀豚、虎鲸的亲缘关系最远。In order to amplify the Tristetraproin ( TTP ) gene CDS sequence from Sutai porcine, and carry out genetic evolution analysis. In this experiment, a pair of specific primers were designed according to Sus scorfa TTP gene CDS sequence published on GenBank.The TTP gene was amplified from Sutai procine blood by RT-PCR and carried out clone and genetic evolution. Results:that the length of the CDS of the amplified TTP gene was 981 bp, encoding a total of 326 amino acids. The homology analysis showed:that the homology of the nucleotide coding sequence of this gene was the highest with the Sus scorfa published on GenBank, which was 99.8%. The phylogenetic tree shows:that the gene and the Sus scrofa are in the same branch of the genetic evolution tree, and the genetic evolution relationship is the closest, and the genetic evolution relationship between the Lipotes vexillifer and the Orcinus orca are the furthest.

关 键 词：苏太猪 TTP基因 克隆 遗传进化分析 

分 类 号：S828.8[农业科学—畜牧学] Q785[农业科学—畜牧兽医]