寄主抗性与黄龙病寄主植物次生化合物相关性初步探讨  

Preliminary discussion the correlation between host resistance and plant secondary compounds of huanlgongbing disease

作　　者：卢慧林 梁广文[2] 李周文婷 欧阳革成 LU Hui-Lin;LIANG Guang-Wen;LIZHOU Wen-Ting;OUYANG Ge-Cheng(Guangdong Key Laboratory of Animal Conservation and Resource Utilization,Guangdong Public Laboratory of Wild Animal Conservation and Utilization,Guangdong Institute of Applied Biological Resources,Guangzhou 510260,China;South China Agricultural University,Guangzhou 510642,China;Guangzhou Science & Technology Exchange Institute,Guangzhou 510115,China)

机构地区：[1]广东省生物资源应用研究所,广东省动物保护与资源利用重点实验室,广东省野生动物保护与利用公共实验室,广州510260 [2]华南农业大学农学院,广州510642 [3]广州市科学技术交流馆,广州510115

出　　处：《环境昆虫学报》2019年第5期1099-1106,共8页Journal of Environmental Entomology

基　　金：广东省科学院发展专项资金项目(2019GDASYL-0105047);广东省科学院科技发展专项(2017GDASCX-0107);国家自然科学基金(31572013)

摘　　要：本文通过使用甲醇浸泡浸提和气相色谱-质谱连用(GC-MS)方法,对黄龙病易感寄主椪柑 Citrus reticulata Blanco cv.Ponkan的健叶、病叶以及隐症寄主九里香 Murraya paniculata (L.) Jack健叶中部分次生化合物的种类及相对含量进行了差异分析。结果显示:椪柑的健康叶片鉴定出32种次生化合物,感病叶片有31种,九里香健康叶片有21种。其中脂肪酸类和简单芳香族类化合物在3样本之间未显示差异;烷烃类化合物在椪柑健叶中含量较高,椪柑病叶和九里香健叶中相对较少;萜类、黄酮类和香豆素类化合物在椪柑健病间的含量相差无几,其中萜类和黄酮类显著多于九里香,而香豆素类则相反。萜类化合物α-石竹烯在椪柑健叶中未检出,但在其病叶和九里香健叶之间无显著差异,此外九里香中特有的萜类有α-姜黄烯(0.15%)、左旋-姜烯(1.00%)、β-倍半水芹烯(0.26%);特有且含量较高的香豆素类化合物主要为蛇床子素(13.97%);而黄酮类化合物在九里香中未检出。两种寄主植物之间以及同种植物在感病与不感病的状态下,次生化合物的种类及相对含量均有显著差异,并初步探讨了其与寄主抗性的相关性。In this study the secondary compounds were extracted from the healthy and diseased leaves of huanglongbing-sensitive mandarin Citrus reticulata Blanco cv Ponkan and the healthy leaves of the disease hidden Murraya paniculata ,using methanol immersion method,and their categories and contents analysed initially by GC-MS.The results showed that 32 and 31 secondary compounds from healthy and diseased C.reticulata leaves,while 21 from healthy M.panicalata leaves were detected,respectively.There were not significantly different in contents of both fatty acid and simple aromatics among the three examined samples.Higher alkane content was detected in healthy C.reticulata leaves while few in both diseased C.reticulata and healthy M.paniculata leaves.Contents of terpenes,flavonids and coumarins were no significant difference between healthy and diseased C.reticulata leaves;the contents of terpenes and flavonoids in both healthy and diseased C.reticulata leaves were significantly higher than M.paniculata leaves,and contrarily,coumarines in the latter was higher than the former.Among them,α-caryophyllen was no significantly different between diseased C.reticulata leaves and M.paniculata leaves,but not detected in healthy C.reticulata leaves.In addition,the special terpenes in M.paniculata leaves were α-Curcumene (0.15%),(-)-Zingiberene (1.00%),and β-sesquiphellandrene (0.26%),and special coumarines in it was mainly osthole (13.97%),and however the flavonoids were not detected.These results demonstrate that the types and contents of the secondary compounds are related to the host plants and their infection status,and the correlation between them and host resistance was chief discussion.

关 键 词：黄龙病 椪柑 九里香 次生化合物 气相色谱-质谱仪连用 寄主抗性 

分 类 号：Q968.1[生物学—昆虫学] S433.39[农业科学—农业昆虫与害虫防治]